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sample preparation	The boiled coffee samples were extracted and filtered with a 0.45 μm Millex syringe filter.
plate	HPTLC Silica gel 60 F254, 20 cm x 10 cm (1.05642.0001)
eluent	[A] acetonitrile; [B] methanol; [C] n-Heptane; (6:4:1, A:B:C)
migration distance	5 cm
sample	0.1 μL to 2.0 μL
detector	UV, 254 nm

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Analysis Note

\r\n

Abstract: HPTLC (High Performance Thin Layer Chromatography) is a very useful analytical technique with very low sample preparation. Shown in the example: the quantification of caffeine in different coffee samples. The boiled coffee samples
\r\nwere extracted and filtered with a 0.45 μm syringe filter. The plate was pre-conditioned with the mobile phase. After chromatographic separation the plate was scanned with a TLC-Scanner and subsequently quantified.
\r\n
\r\nResults: Caffeine was detected under UV light (254 nm, hRf = 56, Fig. 1), (quantification information Fig. 2). A triple determination of each sample is possible in parallel.
\r\n
\r\nConclusion: These results demonstrate that caffeine can be easily quantified by TLC with a three-fold determination and minimal sample preparation.

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