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LP02 Anti-LDL Receptor Mouse mAb (15C8)

This Anti-LDL Receptor Mouse mAb (15C8) is validated for use in ELISA, Flow Cytometry, Frozen Sections, Immunoblotting, Immunocytochemistry, Immunoprecipitation for the detection of LDL Receptor.

Anti-LDL Receptor Mouse mAb (15C8): SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
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      Übersicht

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      B, HMMonoclonal Antibody
      Description
      OverviewRecognizes the ~160 kDa LDL receptor protein in HT1080 and HepG2 cells.
      Catalogue NumberLP02
      Brand Family Calbiochem®
      Application Data
      Detection of human LDL receptor by immunocytochemistry. Sample: HepG2 cells fixed in methanol. Primary antibody: Anti-LDL Receptor Mouse mAb (15C8) (Cat. No. LP02) (5 µg/ml). Detection: fluorescence.
      References
      Referencesvan Driel, I.R., et al. 1987. J. Biol. Chem. 262, 16127.
      van Driel, I.R., et al. 1987. J. Biol. Chem. 262, 17443.
      Russell, D.W., et al. 1984. Cell 37, 577.
      Achneider, W.J., et al. 1983. J. Cell Biol. 97, 1635.
      Cummings, R.D., et al. 1983. J. Biol. Chem. 258, 15261.
      Schneider, W.J., et al. 1982 J. Biol. Chem. 257, 2664.
      Goldstein, J.L., et al. 1979. Nature 279, 679.
      Product Information
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
      Positive controlLiver sections, HT1080 cells, HepG2 cells
      Preservative≤0.1% sodium azide
      Quality SegmentMQ100
      Applications
      Application ReferencesOriginal Clone Russell, D.W., et al. 1984. Cell 37, 577. Specificity van Driel, I.R., et al. 1987. J. Biol. Chem. 262, 16127. Immunoblotting van Driel, I.R., et al. 1987. J. Biol. Chem. 262, 17443. Flow Cytometry Duvillard, L., et al. 2003. Diabetes Care 26, 1540.
      Key Applications Enzyme-Linked Immunosorbent Assay
      Flow Cytometry
      Frozen Sections
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Immunoprecipitation
      Application NotesELISA (0.5-1 µg/ml)
      Flow Cytometry (see application references)
      Frozen Sections (1-5 µg/ml)
      Immunoblotting (1-5 µg/ml, see application references)
      Immunocytochemistry (1-5 µg/ml, IF)
      Immunoprecipitation (1-2 µg/sample)
      Application CommentsWill displace 50-70% of LDL binding to the receptor. For immunoblotting, include 2 mM Ca+2 with the antibody and avoid EDTA. This antibody has been reported to bind best to the unreduced LDL-R (see specificity application reference). Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena trypic digest of bovine low density lipoprotein receptor
      ImmunogenBovine
      Epitopefirst cysteine-rich ligand binding domain
      Clone15C8
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Bovine
      • Human
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade ITEM Number
      Bestellnummer GTIN
      LP02 0

      Documentation

      Anti-LDL Receptor Mouse mAb (15C8) Analysenzertifikate

      TitelChargennummer
      LP02

      Literatur

      Übersicht
      van Driel, I.R., et al. 1987. J. Biol. Chem. 262, 16127.
      van Driel, I.R., et al. 1987. J. Biol. Chem. 262, 17443.
      Russell, D.W., et al. 1984. Cell 37, 577.
      Achneider, W.J., et al. 1983. J. Cell Biol. 97, 1635.
      Cummings, R.D., et al. 1983. J. Biol. Chem. 258, 15261.
      Schneider, W.J., et al. 1982 J. Biol. Chem. 257, 2664.
      Goldstein, J.L., et al. 1979. Nature 279, 679.
      Datenblatt

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision12-June-2008 JSW
      ApplicationELISA (0.5-1 µg/ml)
      Flow Cytometry (see application references)
      Frozen Sections (1-5 µg/ml)
      Immunoblotting (1-5 µg/ml, see application references)
      Immunocytochemistry (1-5 µg/ml, IF)
      Immunoprecipitation (1-2 µg/sample)
      Application Data
      Detection of human LDL receptor by immunocytochemistry. Sample: HepG2 cells fixed in methanol. Primary antibody: Anti-LDL Receptor Mouse mAb (15C8) (Cat. No. LP02) (5 µg/ml). Detection: fluorescence.
      DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with Sp2/0-Ag14 myeloma cells (see application references). Recognizes the ~160 kDa LDL Receptor protein.
      BackgroundThe low density lipoprotein receptor is responsible for mediating the uptake of LDL, a cholesterol carrying plasma lipoprotein. The receptor is a member of a class of cell surface proteins which are concentrated in clathrin coated pits and carry external ligands into cells by receptor-mediated endocytosis. The purified receptor is a glycoprotein with a molecular weight of 160,000. It is synthesized as a precursor of molecular weight 120,000 that is then glycosylated to the mature form by addition of N-linked and O-linked sugars in the Golgi apparatus. Unlike other hepatic lectins, the orientation of the LDL-R is such that the amino terminus is to the exterior of the cell. It has recently been determined that the LDL-R has sequence homology with the epidermal growth factor receptor precursor.
      HostMouse
      Immunogen speciesBovine
      Immunogena trypic digest of bovine low density lipoprotein receptor
      Epitopefirst cysteine-rich ligand binding domain
      Clone15C8
      IsotypeIgG2a
      Speciesbovine, human, not rabbit
      Positive controlLiver sections, HT1080 cells, HepG2 cells
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsWill displace 50-70% of LDL binding to the receptor. For immunoblotting, include 2 mM Ca+2 with the antibody and avoid EDTA. This antibody has been reported to bind best to the unreduced LDL-R (see specificity application reference). Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      Referencesvan Driel, I.R., et al. 1987. J. Biol. Chem. 262, 16127.
      van Driel, I.R., et al. 1987. J. Biol. Chem. 262, 17443.
      Russell, D.W., et al. 1984. Cell 37, 577.
      Achneider, W.J., et al. 1983. J. Cell Biol. 97, 1635.
      Cummings, R.D., et al. 1983. J. Biol. Chem. 258, 15261.
      Schneider, W.J., et al. 1982 J. Biol. Chem. 257, 2664.
      Goldstein, J.L., et al. 1979. Nature 279, 679.
      Application referencesOriginal Clone Russell, D.W., et al. 1984. Cell 37, 577. Specificity van Driel, I.R., et al. 1987. J. Biol. Chem. 262, 16127. Immunoblotting van Driel, I.R., et al. 1987. J. Biol. Chem. 262, 17443. Flow Cytometry Duvillard, L., et al. 2003. Diabetes Care 26, 1540.