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05-1461
Sigma-AldrichAnti-Src Family (WTAPE) Antibody, clone 2E8.2
Use Anti-Src Family (WTAPE) Antibody, clone 2E8.2 (Mouse Monoclonal Antibody) validated in WB to detect Src Family (WTAPE) also known as Integrin-linked kinase, 59 kDa serine/threonine-protein kinase.
More>>Use Anti-Src Family (WTAPE) Antibody, clone 2E8.2 (Mouse Monoclonal Antibody) validated in WB to detect Src Family (WTAPE) also known as Integrin-linked kinase, 59 kDa serine/threonine-protein kinase. Less<<
SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
pp60c-Src (Src) is the prototype non-receptor tyrosine kinase Src was the first proto-oncogenic protein discovered. The 9 members of the Src family include Src, Lyn, Fyn, Yes, Lck, Hck, Blk, Fgr, and Yrk. They share common structural characteristics that include an N-terminal anchor sequence that allows the protein to the membrane followed by both an SH3 and an SH2 domain, a catalytic domain, an activation loop, and finally a C-term region that binds to the SH2 domain when phosphorylated (Tyr527 in Src that is phosphorylated by Csk). This binding causes Src to adopt an inactive conformation. Mutation of Tyr527 to phenylalanine removes the SH2-binding target, and results in a constitutively active kinase. A second mutation at the conserved lysine residue at the ATP-binding site abolishes kinase activity, but without Tyr527, the conformation of the protein is open, allowing interaction with endogenous activators. Consequently, this double-mutant exhibits a dominant-negative phenotype. Homology to Src in three regions is shared by a number of proteins, SH1 being the catalytic domain, SH2 being a phospho-tyrosine binding domain, and SH3 being a proline-binding domain.
References
Product Information
Format
Purified
Control
A431 Cell Lysate
Presentation
Protein G purified mouse monoclonal in PBS and 0.05% NaN3.
Use Anti-Src Family (WTAPE) Antibody, clone 2E8.2 (Mouse Monoclonal Antibody) validated in WB to detect Src Family (WTAPE) also known as Integrin-linked kinase, 59 kDa serine/threonine-protein kinase.
Key Applications
Western Blotting
Biological Information
Immunogen
Shared Src family sequence found at the C-term. Amino acid sequence GAKFPIKWTAPE.
Epitope
C-term WTAPE region
Clone
2E8.2
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
Recognizes Src Family Members at the following amino acids: Src:424-435, Lyn:402-413, Fyn:425-436, Yes:431-442, Lck:399-411, Hck:394-405, Blk:394-405, and Fgr: 417-428.
Isotype
IgG3κ
Species Reactivity
Human
Mouse
Rat
Species Reactivity Note
Human and Rat tested. Expected to react with Mouse.
Summary for proto-oncogene tyrosine-protein kinase SRC [Homo sapiens] (accession no. NP_005408) (one example of Src familyproteins containing the immunogen sequence: other members include Src, Lyn, Fyn, Yes, Lck, Hck, Blk, Fgr, and Yrk). The gene for the proto-oncogene tyrosine-protein kinase SRC [Homo sapiens] is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq].
Summary for SRC_HUMAN (accession no. P12931) (one example of Src familyproteins containing the immunogen sequence: other members include Lyn, Fyn, Yes, Lck, Hck, Blk, Fgr, and Yrk). CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. SUBUNIT: Interacts with DDEF1/ASAP1; via the SH3 domain. Interacts with CCPG1 (By similarity). Interacts with CDCP1, PELP1, TGFB1I1 and TOM1L2. Interacts with the cytoplasmic domain of MUC1, phosphorylates it and increases binding of MUC1 with beta-catenin. Interacts with RALGPS1; via the SH3 domain. Interacts with HEV ORF3 protein; via the SH3 domain. PTM: Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. The phosphorylated tail interacts with the SH2 domain thereby repressing kinase activity. SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. SIMILARITY: Contains 1 protein kinase domain. SIMILARITY: Contains 1 SH2 domain. SIMILARITY: Contains 1 SH3 domain.
Molecular Weight
~60 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
Routinely evaluated by Western Blot Western Blot Analysis: This lot detected Src family members at a 2μg/mL dilution in EGF-treated A431 cell lysate resolved via SDS-PAGE and transferred to PVDF (Immobilon-P).
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at 2-8°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance
