STAR ELISA Kits

Millipore’s Upstate STAR (Signal Transduction Assay Reaction) ELISA kits are a fast, sensitive method to detect the relative amounts of total and activated targets through the use of phospho-specific antibodies.

The STAR assays are a solid phase sandwich enzyme linked immunosorbent assay that provides a fast, sensitive method to detect specific levels of signaling targets in whole cell extracts. The STAR 96-microwell clear plates are coated with a specific mouse monoclonal capture antibody. The detection antibody is either a specific rabbit total or phospho-specific antibody that is used to detect the captured target in the wells of the plate. The sensitive enzymatic detection of the sample is measured at absorbance of 450 nm using a standard plate reader.

The entire assay takes less than five hours to complete, with minimal hands-on time. Many of the reagents are supplied in ready-to-use formulations for ease of use and the kit includes a standard that is run as both a positive control and to develop a standard curve.

Kits are available for the detection of a variety of targets (total and phospho-specific state):

• Akt
  
• Akt1
  
• EGFR
  
• ERK 1/2
  
• FAK
  
• GSK-3β
  
• IGF-1R
  
• IκB
  
• IR
  
• IRS1
  
• JNK 1/2
  
• MEK1
  
• Met
  
• p38α
  
• p53
  
• PRAS40
  
• Src