Separation of Angiotensins

Column	Chromolith® Performance RP-8 endcapped, 100-4.6 mm
Mobile phase	A: Acetonitrile/ water 90/ 10 + 0.1% TFA B: 0.1% TFA in water
Gradient	Time/min	%A	%B
	0.0	45	55
	1.0	90	10
	3.0	90	10
Flow rate	2 mL/min
Pressure	30 - 40 bar
Detection	214 nm
Temperature	Ambient
Injection volume	30 μL
Sample	1. (Sar1, Ala8)-Angiotensin II 2. (Sar1, Ile8)-Angiotensin II 3. Angiotensin		87 μg/mL 87 μg/mL 47 μg/mL