Separation of Acids

Column	Chromolith® Performance RP-8 endcapped, 100-4.6 mm
Mobile phase	A: Acetonitrile B: 20mM NaH2PO4 pH 2.5
Gradient	Time/min 0 0.5 8.5 9.1 16	% A 2 18 18 32 32
Flow rate	1 mL/min
Pressure	23 bar
Detection	220 nm
Temperature	Ambient
Injection volume	5 μL
Sample	1. Malic acid 2. Succinic acid 3. Glutaric acid 4. 3,4-Dihydroxy-cinnamic acid 5. 4-Hydroxy-cinnamic acid 6. Sorbic acid 7. Benzoic acid 8. 2-Hydroxybenzoic acid 9. Cinnamic acid	0.92 mg/mL 1.70 mg/mL 1.20 mg/mL 0.02 mg/mL 0.03 mg/mL 0.20 mg/mL 0.04 mg/mL 0.15 mg/mL 0.04 mg/mL