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AB9744 Anti-14-3-3 τ (tau) Antibody

Detect 14-3-3 τ (tau) using this Anti-14-3-3 τ (tau) Antibody validated for use in ELISA, WB, IH.

Anti-14-3-3 τ (tau) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Neurodegenerative Diseases Gene Symbol: YWHAQ, HS1, 14-3-3, 1C5 UniProt Number: P27348
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AB9744
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, Ch, H, M, R, Rb, ShELISA, WB, IHCRbSerumPolyclonal Antibody
      Description
      Catalogue NumberAB9744
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-14-3-3 τ (tau) Antibody
      Background InformationMammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers, thus rendering 14-3-3 as a key multifunctional regulatory molecule. 14-3-3 isomers have been implicated in several neurological disorders: for example isoforms gamma, epsilon and beta are expressed in the cerebrospinal fluid of Creutzfeldt-Jakob patients, but not patients suffering from other neurological conditions such as Alzheimer's.

      The 14-3-3 tau antibody is one of a panel of antibodies that were generated against 14-3-3 isoforms and have demonstrated their potential for use as an ELISA-based diagnostic tool. These antibodies will allow the research to investigate the role of this protein in key cellular processes and neurological disorders such as Creutzfeldt-Jakob, Alzheimer's and Parkinson's Disease.
      References
      Product Information
      FormatSerum
      PresentationLiquid.
      Quality SegmentMQ100
      Applications
      ApplicationDetect 14-3-3 τ (tau) using this Anti-14-3-3 τ (tau) Antibody validated for use in ELISA, WB, IH.
      Key Applications
      • ELISA
      • Western Blotting
      • Immunohistochemistry
      Application NotesWestern blot: 1:3,000 using ECL.
      Immunohistochemistry: 1:30-1:60 on mouse brain tissue which was treated by hydrated autoclaving at 121°C for 15 minutes prior to immunolabeling. Endogenous peroxidase was blocked with hydrogen peroxide in methanol and non-specific labeling with normal goat serum at 1:20 for 5 minutes.
      ELISA
      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenSynthetic peptide from the N-terminal area of sheep 14-3-3 tau.
      HostRabbit
      Specificity14-3-3 tau (theta). By Western blot the antibody recognizes the ~30 kDa protein.
      Species Reactivity
      • Bovine
      • Chicken
      • Human
      • Mouse
      • Rat
      • Rabbit
      • Sheep
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease.
      Gene Symbol
      • YWHAQ
      • HS1
      • 14-3-3
      • 1C5
      Purification MethodSerum
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P27348 # Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
      SIZE: 245 amino acids; 27764 Da
      SUBUNIT: Interacts with PCTK1 (By similarity). Homodimer. Interacts with SSH1.
      SUBCELLULAR LOCATION: Cytoplasm. Note=In neurons, axonally transported to the nerve terminals.
      TISSUE SPECIFICITY: Abundantly expressed in brain, heart and pancreas, and at lower levels in kidney and placenta. Up-regulated in the lumbar spinal cord from patients with sporadic amyotrophic lateral sclerosis (ALS) compared with controls, with highest levels of expression in individuals with predominant lower motor neuron involvement.
      SIMILARITY: SwissProt: P27348 ## Belongs to the 14-3-3 family.
      Molecular Weight30 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade ITEM Number
      Catalogue Number GTIN
      AB9744 04053252509254

      Documentation

      Anti-14-3-3 τ (tau) Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-14-3-3 τ (tau) Antibody Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-14-3-3 tau - 3277978 3277978
      RABBIT ANTI-14-3-3 tau POLYCLONAL ANTIBODY 2476711

      References

      Reference overviewPub Med ID
      Interaction of Akt-phosphorylated ataxin-1 with 14-3-3 mediates neurodegeneration in spinocerebellar ataxia type 1
      Chen, Hung-Kai, et al
      Cell, 113:457-68 (2003)  2003

      		12757707 12757707
      Immunolocalisation of 14-3-3 isoforms in normal and scrapie-infected murine brain
      Baxter, H C, et al
      Neuroscience, 109:5-14 (2002)  2002

      		11784696 11784696
      Identification of 14-3-3 proteins in human platelets: effects of synthetic peptides on protein kinase C activation
      Wheeler-Jones, C P, et al
      Biochem J, 315 ( Pt 1):41-7 (1996)  1996

      		8670130 8670130