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Durapore® Membrane Filters

Durapore® membranes made with Polyvinylidene fluoride (PVDF) provide high flow rates and throughput, low extractables and broad chemical compatibility.

Overview

Specifications

Ordering Information

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Hydrophilic Durapore Membrane Filters · Catalogue No.Clear Sorting & Filtering Show Filter
Catalogue NumberPore SizeFilter Diameter (⌀)DimensionsPack Size
VVLP013000.1 µm 13 mm PVDF, Hydrophilic, 0.1 µm, 13 mm, white, plain, 100 Show Pricing & Availability
VVLP025000.1 µm 25 mm PVDF, Hydrophilic, 0.1 µm, 25 mm, white, plain, 100 Show Pricing & Availability
VVLP047000.1 µm 47 mm PVDF, Hydrophilic, 0.1 µm, 47 mm, white, plain, 100 Show Pricing & Availability
VVLP062250.1 µm 63.5 mm PVDF, Hydrophilic, 0.1 µm, 63.5 mm, white, plain, 25 Show Pricing & Availability
VVLP076250.1 µm 76 mm PVDF, Hydrophilic, 0.1 µm, 76 mm, white, plain, 25 Show Pricing & Availability
VVLP090500.1 µm 90 mm PVDF, Hydrophilic, 0.1 µm, 90 mm, white, plain, 50 Show Pricing & Availability
VVLP142500.1 µm 142 mm PVDF, Hydrophilic, 0.1 µm, 142 mm, white, plain, 50 Show Pricing & Availability
GVWP000100.22 µm Not Applicable 1 roll x 10ft PVDF, Hydrophilic, 0.22 µm white, plain, 1 roll x 10ft Show Pricing & Availability
GVWP013000.22 µm 13 mm PVDF, Hydrophilic, 0.22 µm, 13 mm, white, plain, 100 Show Pricing & Availability
GVWP025000.22 µm 25 mm PVDF, Hydrophilic, 0.22 µm, 25 mm, white, plain, 100 Show Pricing & Availability
GVWP047000.22 µm 47 mm PVDF, Hydrophilic, 0.22 µm, 47 mm, white, plain, 100 Show Pricing & Availability
GVWP062250.22 µm 63.5 mm PVDF, Hydrophilic, 0.22 µm, 63.5 mm, white, plain, 25 Show Pricing & Availability
GVWP076250.22 µm 76 mm PVDF, Hydrophilic, 0.22 µm, 76mm, white, plain, 25 Show Pricing & Availability
GVWP090500.22 µm 90 mm PVDF, Hydrophilic, 0.22 µm, 90 mm, white, plain, 50 Show Pricing & Availability
GVWP100500.22 µm 100 mm PVDF, Hydrophilic, 0.22 µm, 100 mm, white, plain, 50 Show Pricing & Availability
GVWP142500.22 µm 142 mm PVDF, Hydrophilic, 0.22 µm, 142 mm, white, plain, 50 Show Pricing & Availability
HVLP000100.45 µm Not Applicable PVDF, Hydrophilic, 0.45 µm, white, plain, 1 roll x 10ft Show Pricing & Availability
HVLP013000.45 µm 13 mm PVDF, Hydrophilic, 0.45 µm, 13 mm, white, plain, 100 Show Pricing & Availability
HVLP025000.45 µm 25 mm PVDF, Hydrophilic, 0.45 µm, 25 mm, white, plain, 100 Show Pricing & Availability
HVLP047000.45 µm 47 mm PVDF, hydrophilic, 0.45 µm, 47 mm, white, plain, 100 Show Pricing & Availability
HVLP062250.45 µm 63.5 mm PVDF, Hydrophilic, 0.45 µm, 63.5 mm, white, plain, 25 Show Pricing & Availability
HVLP076250.45 µm 76 mm PVDF, Hydrophilic, 0.45 µm, 76mm, white, plain, 25 Show Pricing & Availability
HVLP090500.45 µm 90 mm PVDF, Hydrophilic, 0.45 µm, 90 mm, white, plain, 50 Show Pricing & Availability
HVLP142500.45 µm 142 mm PVDF, Hydrophilic, 0.45 µm, 142 mm, white, plain, 50 Show Pricing & Availability
HVWG047000.45 µm 47 mm Hydrophilic, 0.45 µm, 47 mm, white, gridded, 100 Show Pricing & Availability
DVPP000100.65 µm Not Applicable PVDF, Hydrophilic, 0.65 µm white, plain, 1 roll x 10ft Show Pricing & Availability
DVPP013000.65 µm 13 mm PVDF, Hydrophilic, 0.65 µm, 13 mm, white, plain, 100 Show Pricing & Availability
DVPP025000.65 µm 25 mm PVDF, Hydrophilic, 0.65 µm, 25 mm, white, plain, 100 Show Pricing & Availability
DVPP047000.65 µm 47 mm PVDF, Hydrophilic, 0.65 µm, 47 mm, white, plain, 100 Show Pricing & Availability
DVPP082500.65 µm 82 mm PVDF, Hydrophilic, 0.65 µm, 82 mm, white, plain, 50 Show Pricing & Availability
DVPP090500.65 µm 90 mm PVDF, Hydrophilic, 0.65 µm, 90 mm, white, plain, 50 Show Pricing & Availability
DVPP142500.65 µm 142 mm PVDF, Hydrophilic, 0.65 µm, 142 mm, white, plain, 50 Show Pricing & Availability
DVPP293250.65 µm 293 mm 0.65um 293mm, 25/pack Show Pricing & Availability
SVLP013005.0 µm 13 mm PVDF, Hydrophilic, 5.0 µm, 13 mm, white, plain, 100 Show Pricing & Availability
SVLP025005.0 µm 25 mm PVDF, Hydrophilic, 5.0 µm, 25 mm, white, plain, 100 Show Pricing & Availability
SVLP047005.0 µm 47 mm PVDF, Hydrophilic, 5.0 µm, 47 mm, white, plain, 100 Show Pricing & Availability
SVWG047005.0 µm 47 mm PVDF, Hydrophilic, 5.0 µm, 47 mm, white, gridded, 100 Show Pricing & Availability
SVLP090505.0 µm 90 mm PVDF, Hydrophilic, 5.0 µm, 90 mm, white, plain, 50 Show Pricing & Availability

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Hydrophobic Durapore Membrane FiltersClear Sorting & Filtering Show Filter
Catalogue NumberPore SizeFilter Diameter (⌀)Pack Size
VVHP047000.1 µm 47 mm PVDF, hydrophobic, 0.1 µm, 47 mm, white, plain, 100 Show Pricing & Availability
GVHP000100.22 µm Not Applicable PVDF, hydrophobic, 0.22 µm, white, plain, 1 roll x 10ft Show Pricing & Availability
GVHP013000.22 µm 13 mm PVDF, hydrophobic, 0.22 µm, 13 mm, white, plain, 100 Show Pricing & Availability
GVHP025000.22 µm 25 mm PVDF, hydrophobic, 0.22 µm, 25 mm, white, plain, 100 Show Pricing & Availability
GVHP047000.22 µm 47 mm PVDF, hydrophobic, 0.22 µm, 47 mm, white, plain, 100 Show Pricing & Availability
GVHP090500.22 µm 90 mm PVDF, hydrophobic, 0.22 µm, 90 mm, white, plain, 50 Show Pricing & Availability
GVHP142500.22 µm 142 mm PVDF, hydrophobic, 0.22 µm, 142 mm, white, plain, 50 Show Pricing & Availability
HVHP013000.45 µm 13 mm PVDF, hydrophobic, 0.45 µm, 13 mm, white, plain, 100 Show Pricing & Availability
HVHP025000.45 µm 25 mm PVDF, hydrophobic, 0.45 µm, 25 mm, white, plain, 100 Show Pricing & Availability
HVHP047000.45 µm 47 mm PVDF, hydrophobic, 0.45 µm, 47 mm, white, plain, 100 Show Pricing & Availability
HVHP090500.45 µm 90 mm PVDF, hydrophobic, 0.45 µm, 90 mm, white, plain, 50 Show Pricing & Availability
HVHP142500.45 µm 142 mm PVDF, hydrophobic, 0.45 µm, 142 mm, white, plain, 50 Show Pricing & Availability

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AccessoryClear Sorting & Filtering Show Filter
Catalogue NumberDescriptionPack Size
XX6200006PFilter forceps, blunt end, stainless steel 3 Show Pricing & Availability

* The membrane disc SVWG 047 00 has a gridded surface

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Documentation

References

Reference overview
Colony filtration blot: a new screening method for soluble protein expression in Escherichia coli
Tobias Cornvik, Sue-Li Dahlroth, Audur Magnusdottir, Maria Dolores Herman, Rosemarie Knaust, Monica Ekberg & Pär Nordlund
Nature Methods, 2005 Jul:2(7):507-509  2005

A functional study on polymorphism of the ATP-binding Cassette transpoter ABCG2:
Toshihisa Ishikawa,Biochem.J, 373, 767-774, 2003, internal ref ABC-02
Biochem.J, 373, 767-774, 2003, internal ref ABC-02  2003

Comparison of microporous membrane morphologies using confocal scanning laser microscopy
Charcosset C (reprint), et al.
Journal of membrane science. 2000. v168, n2, p53-62  2000

ATP/Mg2+-dependent Cardiac Transport system for Flutathione S-Conjugates
Toshihisa Ishikawa,J.Biological Chemistry, 264:29, 17343-17348, 1989, internal ref ABC-01
J.Biological Chemistry, 264:29, 17343-17348, 1989, internal ref ABC-01  1989

FAQ

QuestionAnswer
How do I tell the membrane from the separator?The blue paper is the separator.
How do I assemble a diffusion chamber containing tissue for implantation?
  1. Wet a single membrane filter (either GSWP 013 00 or HAWP 013 00) with Milli-Q water and blot it to absorbent paper to remove excess water.
  2. Glue the filter to one side of a diffusion chamber ring without hole (catalog number PR00 014 00), using MF cement (catalog number XX70 000 00), and let dry.
  3. Sterilize this assembly, along with another membrane filter of the same type, by exposure to ethylene oxide gas or ultraviolet light.
  4. Moisten the half-completed chamber and the separate sterile filter with a sterile physiological solution, and aseptically place the tissue to be studied in the half-completed chamber assembly.
  5. Glue the cover filter in place using sterile technique.
What are the dimensions and width of the grids on the Millipore 47mm gridded membranes?The grids are 3.08mm x 3.08mm square. A grid line is 44um in width and there is are average of 169 gridded squares per filter. Please note however that the actual filtration area and therefore the total number of squares in that area is dependent on the filter holder. For filter holders with 9.6sq.cm of filtration area there are 100 squares available. For filter holders with 13.8 sq.cm of filtration area the number of available grids are approximately 140.
How do I assemble a diffusion chamber containing suspension cells for implantation?Wet two membrane filters (either GSWP 013 00 or HAWP 013 00) with Milli-Q water and blot them to absorbent paper to remove excess water. Glue a moist filter to each side of the diffusion chamber ring with hole (catalog number PR00 014 01) using MF cement (catalog number XX70 000 00). Sterilize the dried diffusion chamber with ethylene oxide gas or ultraviolet light. After sterilization, inject the cells through the hole in the plastic ring, and plug the hole with the nylon thread which comes with the rings (catalog number PR00 000 00 if ordered separately).
Can Millipore filters remove mycoplasma from my solution?Mycoplasma can be removed from solutions by using a 0.1 um pore size filter. For mycoplasma reduction the 0.1 um Durapore filter can be used. Its Millipore code is VV. A 0.1 um Express membrane can also be used for removal of mycoplasma. Its membrane code is VP. These membranes are available in a number of fabricated fiter devices. For help determining which device would be best for your solution, call Millipore Technical Services.
Are the pore sizes of your Durapore membranes uniform or asymmetric?The surfaces of the Durapore membrane are symmetric, however, the actual pores are not straight-through holes but rather follow a torturous path.
Which membrane should I use to filter my solution?For general filtration use MCE. When you are looking for the lowest protein binding membrane use Durapore. For speed, and when filtering serum solutions use Millipore Express.
I would like to use the Durapore membrane discs and am interested in knowing what is meant by average pore size?Since the size and uniformity of the Durapore membrane is accurately controlled during manufacturing, it is possible to rate membranes as absolute filters based on the largest particle that can pass through the membrane. It is then possible to confirm the pore size rating of the membrane filter using a non-destructive integrity test referred to as the bubble point test. This test is based on the fact that liquid is held in the membrane pores by surface tension and that the minimum pressure required to force liquid from the capillary structure is directly related to the capillary diameter.
Which side of the membrane should I use, the shiny or dull side?Most researchers may not even notice that there is a "sidedness" to filters, and, for most applications, orientation will not affect filter performance. However, membranes do have a slightly asymmetric pore structure: the shiny side of the membrane is the "tighter" side. In some applications, you can take advantage of this difference by selecting a specific filter orientation. ultrafiltration membranes should always be used shiny side up, regardless of application for drop dialysis ( a buffer exchange technique in which a few drops of DNA or protein are placed in a 0.05 or 0.025 um filter and floated on a buffer solution), apply the sample to the shiny side of the filter and float the filter dull side to the buffer. This measure will enhance buffer exchange and discourage sample loss. The Millipore Express and Express Plus membranees are also sided - these membranes should be used shiny side facing down.
I have a dilute protein solution. I want to make sure I do not lose any of my protein. Which filter should I use?You should use any device that contains a Durapore filter. Durapore is the lowest protein-binding filter available. This filter will give the best yield when filtering a dilute protein solution.
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