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GF142 Platelet Derived Growth Factor-AA Protein, Recombinant human

The Platelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle & connective tissue.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Cell Culture UniProt Number: P04085 Entrez Gene Number: NM_002607.4, NM_033023.3
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GF142
10 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Key ApplicationsSpeciesSourcePurity
      CULTHuman E.coliGreater than 98% by SDS-PAGE and HPLC analysis.
      Description
      Catalogue NumberGF142
      ReplacesGF017
      DescriptionPlatelet Derived Growth Factor-AA Protein, Recombinant human
      OverviewPlatelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle and connective tissue. PDGF, which is composed of a dimer of two chains termed the A chain and B chain, can be present as AA or BB homodimers or as an AB heterodimer. Human PDGF-AA is a 28.5 kDa A chain homodimer protein containing 250 amino acid residues.
      Alternate Names
      • PDGF-AA
      References
      Product Information
      PresentationLyophilized from ddH2O.
      Quality SegmentMQ100
      Applications
      ApplicationThe Platelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle & connective tissue.
      Key Applications
      • Cell Culture
      Application NotesFor most in vitro applications, PDGF-AA exerts its biological activity in the concentration range of 0.2 to 10.0 ng/mL. Responding cells are (partial list): Fibroblasts, connective tissue.
      Biological Information
      PurityGreater than 98% by SDS-PAGE and HPLC analysis.
      SourceE.coli
      Specific ActivityHuman PDGF-AA is fully biologically active when compared to standards, as determined by colorimetric assay using BALB / 3T3 cells.
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer or as a heterodimer with the platelet-derived growth factor beta polypeptide, where the dimers are connected by disulfide bonds. Studies using knockout mice have shown cellular defects in oligodendrocytes, alveolar smooth muscle cells, and Leydig cells in the testis; knockout mice die either as embryos or shortly after birth. Two splice variants have been identified for this gene.
      Gene Symbol
      • PDGFA
      • PDGF-1
      • PDGF-A
      • PDGF1
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P04085 # Platelet-derived growth factor is a potent mitogen for cells of mesenchymal origin. Binding of this growth factor to its affinity receptor elicits a variety of cellular responses. It is released by platelets upon wounding and plays an important role in stimulating adjacent cells to grow and thereby heals the wound.
      SIZE: 211 amino acids; 24043 Da
      SUBUNIT: Antiparallel disulfide-linked dimer of non-identical (A and B) chains. Homodimers of A and B chains are implicated in transformation processes. Interacts with CSPG4.
      SUBCELLULAR LOCATION: Secreted.
      DOMAIN: SwissProt: P04085 The long form contains a basic insert which acts as a cell retention signal.SIMILARITY: Belongs to the PDGF/VEGF growth factor family.
      MISCELLANEOUS: A-A and B-B, as well as A-B, dimers can bind to the PDGF receptor.
      Stem Cell Type
      • Neural Stem Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsThe lyophilized PDGF-AA is stable for a few weeks at room temperature, but best stored at -80°C. After a quick spin, reconstitute in ddH2O to a concentration of 0.1-1.0 mg/mL. This solution can be diluted into aqueous buffered solutions and stored at 2-8°C for one week or -20°C for future use. Reconstituted PDGF-AA should be stored in undiluted aliquots at -20°C for up to six months.
      Packaging Information
      Material Size10 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade ITEM Number
      Catalogue Number GTIN
      GF142 04053252271618

      Documentation

      Platelet Derived Growth Factor-AA Protein, Recombinant human SDS

      Title

      Safety Data Sheet (SDS) 

      Platelet Derived Growth Factor-AA Protein, Recombinant human Certificates of Analysis

      TitleLot Number
      PDGF-aa, Human Recombinant 3070095
      PDGF-aa, Human Recombinant 2823625
      PDGF-aa, Human Recombinant 2944660
      PDGF-aa, Human Recombinant 3140342
      PDGF-aa, Human Recombinant - 3278858 3278858
      PDGF-aa, Human Recombinant - 3660741 3660741
      PDGF-aa, Human Recombinant - 4026086 4026086
      PDGF-aa, Human Recombinant - 4202016 4202016
      PDGF-aa, Human Recombinant - 4304047 4304047
      PDGF-aa, Human Recombinant - 4350046 4350046
      Show more

      Technical Info

      Title
      Derivation of oligodendrocyte progenitor cells from a human neural stem cell line

      Data Sheet

      Title
      PDGF-aa, Human Recombinant
      Reprogramming Cell Fate and Function Novel Strategies for iPSC Generation, Characterization, and Differentiation

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      Categories

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      Life Science Research > Cell Culture and Systems > Cell Culture Media and Supplements > Cell Culture Supplements > Cytokines & Growth Factors