71230 MilliporeLysonase™ Bioprocessing Reagent

Lysonase™ Bioprocessing Reagent is an optimized, ready-to-use blend of rLysozyme™ Solution and Benzonase Nuclease. rLysozyme Solution contains a highly purified and stabilized recombinant lysozyme with specific activity 250 times greater than that of chicken egg white lysozyme. Benzonase Nuclease is a genetically engineered nonspecific endonuclease that degrades all forms of DNA and RNA (single stranded, double stranded, circular, linear), reducing extract viscosity and increasing protein yield. The combined activities of rLysozyme and Benzonase Nuclease significantly increase protein extraction efficiency and facilitate downstream processing of protein extracts. For efficient protein extraction with BugBuster^® Protein Extraction Reagent, use 10 µl Lysonase per 1 g cell paste. For efficient protein extraction with PopCulture^® Reagent, add 2 µl Lysonase per 1 ml culture. Store at –20°C.