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MABS1287 Anti-EPO-R Antibody, clone VP-2E8

Anti-EPO-R, clone VP-2E8, Cat. No. MABS1287, is a mouse monoclonal antibody that detects erythropoietin receptor and is tested for use in Flow Cytometry, Immunocytochemistry, Immunofluorescence, and Immunoprecipitation.

Anti-EPO-R Antibody, clone VP-2E8: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Research Category: Signaling Gene Symbol: EPOR UniProt Number: P19235
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MABS1287
100 μg  
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      Übersicht

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFC, ICC, IF, IPMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS1287
      DescriptionAnti-EPO-R Antibody, clone VP-2E8
      Alternate Names
      • Erythropoietin Receptor
      Background InformationErythropoietin receptor (UniProt: P19235, also known as EPO-R) is encoded by the EPOR gene (Gene ID: 2057) in human. EPO-R is a single-pass type I membrane protein that serves as a receptor for erythropoietin. It mediates erythropoietin-induced erythroblast proliferation and differentiation. Upon EPO stimulation, EPO-R dimerizes triggering the JAK2/STAT5 signaling cascade. EPO-R has an extracellular domain (aa 25-250), a transmembrane domain (aa 251-273), and a cytoplasmic domain (aa 274-508). It is synthesized with a signal peptide (aa 1-24) that is cleaved to generate mature EPO-R. Upon stimulation following Erythropoietin (EPO) binding, EPO-R is phosphorylated on C-terminal tyrosine residues by JAK2. The phosphotyrosine motifs are also recruitment sites for several SH2-containing proteins and adapter proteins, which mediate cell proliferation. Phosphorylation on Tyrosine 454 is shown to be essential for PTPN6 interaction, and on Tyrosine 426 for PTPN11 interaction. Tyrosine 426 is also required for SOCS3 binding, however, Tyr-454/Tyr-456 motif is reported to be the preferred binding site. Ubiquitination of EPO-R at Lysine 81 is required for receptor internalization, whereas ubiquitination at Lysine 453 promotes trafficking of activated receptors to the lysosomes for degradation. Defects in EPOR gene are linked to familial erythrocytosis which is characterized by increased serum red blood cell mass, elevated hemoglobin and hematocrit, and hypersensitivity of erythroid progenitors to erythropoietin.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in PBS without preservatives.
      Quality SegmentMQ100
      Applications
      ApplicationAnti-EPO-R, clone VP-2E8, Cat. No. MABS1287, is a mouse monoclonal antibody that detects erythropoietin receptor and is tested for use in Flow Cytometry, Immunocytochemistry, Immunofluorescence, and Immunoprecipitation.
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      • Immunofluorescence
      • Immunoprecipitation
      Application NotesFlow Cytometry Analysis: 2 µg/mL from a representative lot detected EPO-R in UT-7 (EPO dependent) cells.

      Immunoprecipitation Analysis: A representative lot detected EPO-R in Immunoprecipitation applications (Maxwell, P., et. al. (2015). Br J Haematol. 168(3):429-42).

      Immunofluorescence Analysis: A representative lot detected EPO-R in Immunofluorescence applications (Maxwell, P., et. al. (2015). Br J Haematol. 168(3):429-42).

      Flow Cytometry Analysis: A representative lot detected EPO-R in Flow Cytometry applications (Maxwell, P., et. al. (2015). Br J Haematol. 168(3):429-42).
      Biological Information
      ImmunogenA recombinant fragment corresponding to the extracellular domain of human EPO-R.
      Epitopeextracellular domain
      CloneVP-2E8
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone VP-2E8 recognizes EPO-R in human cells. It targets an epitope within the extracellular domain.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • EPOR
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight55.06 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in starved UT-7 cells.

      Immunocytochemistry Analysis: 1:500 dilution of this antibody detected EPO-R in starved UT-7 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.

      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade ITEM Number
      Bestellnummer GTIN
      MABS1287 04054839328732