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SCT071 BioTracker BacGO Gram-positive Bacteria Dye

Live cell imaging fluorescent probe for detection of gram-posotive bacteria in vitro, in vivo and in bacterial sludge

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SCT071
1 mg  
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      Übersicht

      Replacement Information
      Description
      Catalogue NumberSCT071
      DescriptionBioTracker BacGO Gram-positive Bacteria Dye
      OverviewGram staining has been the gold standard technique for bacterial classification based on the different physical properties of the bacterial cell‐wall structure. However, Gram staining requires several steps, that is, fixation of the bacterial cell, two steps of staining with two different dyes, followed by decolorization, and washing. Notably, the decolorization step often generates false Gram determination, and the washing step can cause significant cell loss. An alternative to the Gram staining method is a fluorescence‐based dye approach for flow cytometry or fluorescence microscopy.

      BioTracker BacGO Gram-positive Bacteria Dye is an orange colored dye that can be used to identify Gram-positive bacteria by flow cytometry and fluorescence imaging. BacGO dye has been tested using real samples of colony‐forming bacteria, activated bacterial sludge, and a bacterial eye infection model. To fully demonstrate the universal selectivity of BacGO dye, it was tested on 16 bacterial strains (9 Gram‐positive and 7 Gram-negative) and compared the results with Gram staining. BacGO stained all of the known Gram‐positive bacteria and the result was validated with Gram staining. The universal selectivity of BacGO was also confirmed by flow cytometry using DAPI to stain the nuclei of all the bacteria.

      Spectral properties:
      Excitation: 558 nm
      Emission: 573 nm

      References:
      Kwon H-Y et al. Development of a Universal Fluorescent Probe for Gram-Positive Bacteria. Ang. Chemie Intl Edn. June 17, 2019, 58, 8426-8431.
      Alternate Names
      • Live cell imaging probe
      • Gram-positive bacteria probe
      References
      Product Information
      Detection methodFluorescence
      PresentationLyophilized
      Applications
      ApplicationLive cell imaging fluorescent probe for detection of gram-posotive bacteria in vitro, in vivo and in bacterial sludge
      Key Applications
      • Cell Based Assays
      Application NotesLive cell fluorescent imaging
      Biological Information
      Molecular Weight485.10
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssurancePurity: ≥ 98% confirmed by HPLC. Structure confirmed by HNMR, FNMR, LC-MS and elemental analysis
      Molar Mass: 485.10 g/mol
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore BioTracker BacGO Gram-positive Bacteria Dye at -20°C, desiccate and protect from light
      Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
      Packaging Information
      Material Size1 mg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade ITEM Number
      Bestellnummer GTIN
      SCT071 04061841441052