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48-602MAG
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17-656
Sigma-AldrichChIPAb+ Sox-2, clone 6F1.2 - ChIP Validated Antibody and Primer Set
This ChIPAb+ Sox-2, clone 6F1.2 -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
More>>This ChIPAb+ Sox-2, clone 6F1.2 -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers. Less<<
SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
ChIPAb+ Sox-2, clone 6F1.2 - ChIP Validated Antibody and Primer Set
Alternate Names
Transcription factor SOX-2
SOX2
Background Information
All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction. The ChIPAb+ Sox-2, clone 6F1.2 antibody/primer set includes the Sox-2, clone 6F1.2 antibody, a negative control antibody (purified mouse IgG), and qPCR primers which amplify a 95 bp region within the promoter of the human nanog gene. The Sox-2, clone 6F1.2 antibody and negative control antibodies are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of Sox-2-associated chromatin.
Sox-2 plays a key role in embryonic stem cell development, and has been shown to help stem cells maintain pluripotent potential. It has also been shown to be associated with uncommitted dividing stem cell and precursor cells of the developing CNS.
References
Product Information
Components
Anti-Sox-2, clone 6F1.2 (mouse monoclonal IgG), Part No. CS204373. One vial containing 100 μg of protein A purified antibody in 140 μL of 0.02 M phosphate buffer pH 7.6 with 0.25 M NaCl containing 0.1% sodium azide, and 30% glycerol. Store at -20°C.
Normal Mouse IgG, Part No. CS200621. One vial containing 125 μg of purified mouse IgG in 125 μL storage buffer containing 0.1% sodium azide. Store at -20°C.
ChIP Primers, nanog promoter, Part No. CS204374. One vial containing 75 μL of 5 μM of each primer specific for the promoter region of human nanog. Store at -20°C. FOR: GTT CTG TTG CTC GGT TTT CT REV: TCC CGT CTA CCA GTC TCA CC
Format
Purified
Control
Includes negative control mouse IgG antibody and primers specific for human nanog promoter region.
This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT).
FUNCTION: SwissProt: P48431 # Can form ternary complexes with OCT-1 or OCT-3 but acts as a transcriptional activator of FGF-4 enhancer DNA sequences only in conjunction with OCT-3 (By similarity).
SIZE: 317 amino acids; 34310 Da
SUBCELLULAR LOCATION: Nucleus.
DISEASE: SwissProt: P48431 # Defects in SOX2 are the cause of microphthalmia syndromic type 3 (MCOPS3) [MIM:206900]. MCOPS3 is characterized by the association of microphthalmia or clinical anophthalmia with anomalies such as microcephaly, short stature, hypogonadotropic hypogonadism, esophageal atresia and neurologic manifestations.
Chromatin Immunoprecipitation: Sonicated chromatin prepared from Ntera 2 cells (3 X 106 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 4 µg of either a normal mouse IgG, or Anti-Sox-2, clone 6F1.2 antibody and the Magna ChIP™ G Kit (Cat. No. 17-611). Successful immunoprecipitation of Sox-2-associated DNA fragments was verified by qPCR using ChIP Primers, nanog promoter (Figure 1). Please refer to the EZ-Magna ChIP™ G (Cat. No. 17-409) or EZ-ChIP™ (Cat. No. 17-371) protocol for experimental details.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size
25 assays
Material Package
25 assays per set. Recommended use: ~4 μg antibody per chromatin immunoprecipitation (dependent upon biological context).
Transport Information
Supplemental Information
Specifications
Global Trade ITEM Number
Bestellnummer
GTIN
17-656
04053252284472
Documentation
ChIPAb+ Sox-2, clone 6F1.2 - ChIP Validated Antibody and Primer Set SDB
Although the principles that balance stem cell self-renewal and differentiation in normal tissue homeostasis are beginning to emerge, it is still unclear whether cancer cells with tumour initiating potential are similarly governed, or whether they have acquired distinct mechanisms to sustain self-renewal and long-term tumour growth. Here we show that the transcription factor Sox2, which is not expressed in normal skin epithelium and is dispensable for epidermal homeostasis, marks tumour initiating cells (TICs) in cutaneous squamous cell carcinomas (SCCs). We demonstrate that Sox2 is required for SCC growth in mouse and human, where it enhances Nrp1/Vegf signalling to promote the expansion of TICs along the tumour-stroma interface. Our findings suggest that distinct transcriptional programmes govern self-renewal and long-term growth of TICs and normal skin epithelial stem and progenitor cells. These programmes present promising diagnostic markers and targets for cancer-specific therapies.
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