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FCCS025145 FlowCellect™ PLC-gamma 1 Activation Dual Detection Kit

This FlowCellect PLC-g1 Activation Dual Detection Kit for Flow Cytometry includes 2 conjugated antibodies, a phospho-specific Anti-phospho-PLC-g1 (Tyr837)-Alexa Fluor 488 & an Anti-PLC-g1-PE conjugated antibody to measure total levels of PLC-g1.

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FCCS025145
25 Tests / Kit  
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      Replacement Information
      Description
      Catalogue NumberFCCS025145
      Trade Name
      • FlowCellect
      DescriptionFlowCellect™ PLC-gamma 1 Activation Dual Detection Kit
      OverviewMillipore’s FlowCellect™ PLC-g1 Activation Dual Detection kit includes two directly conjugated antibodies, a phospho-specific Anti-phospho-PLC-g1 (Tyr837)-Alexa Fluor® 488 and an Anti-PLC-g1-PE conjugated antibody to measure total levels of PLC-g1. This two color flow cytometry kit is designed to detect the extent of PLC-g1 pathway activation by measuring PLC-g1 phosphorylation relative to the total PLC-g1 expression in any given cell population. By doing such, the levels of both the total and phosphorylated protein can be measured simultaneously in the same cell, resulting in a normalized and accurate measurement of PLC-g1 activation after stimulation. Moreover, simultaneous measurement of both total and phospho-PLC-g1 confirms target specificity of the phosphorylation event. Together, a total and phospho antibody duo performed in multiplex provides an enhanced and more reliable detection of the phospho: total ratio within a mixed population.

      PLC-g1 is devoted to tyrosine kinase-dependent signaling for mitogenic responses to diverse agents such as growth factor activation of receptor tyrosine kinases and stimulation of immune cell receptors. Signaling pathways that depend on tyrosine kinases communicate with the PLC-g family. Because of its involvement in many key signaling cascades resulting from receptor tyrosine kinase activation, PLC-g1 plays a critical role in tumor progression and cancer-related illnesses. When stimulating A431 cells with EGF for 2 minutes the total protein level remains constant compared to unstimulated cells, while phospho-PLC-g1 levels are increased in all cells indicating that there is no competition between the two antibodies for their target epitopes.
      Alternate Names
      • Flow Cellect
      Background InformationMillipore’s FlowCellect™ PLC-gamma 1 Activation Dual Detection kit includes two directly conjugated antibodies, a phospho-specific Anti-phospho-PLC-gamma 1 (Tyr837)-Alexa Fluor® 488 and an Anti-PLC-gamma 1-PE conjugated antibody to measure total levels of PLC-gamma 1. This two color flow cytometry kit is designed to detect the extent of PLC-gamma 1 pathway activation by measuring PLC-gamma 1 phosphorylation relative to the total PLC-gamma 1 expression in any given cell population. By doing such, the levels of both the total and phosphorylated protein can be measured simultaneously in the same cell, resulting in a normalized and accurate measurement of PLC-gamma 1 activation after stimulation. Moreover, simultaneous measurement of both total and phospho-PLC-gamma 1 confirms target specificity of the phosphorylation event. Together, a total and phospho antibody duo performed in multiplex provides an enhanced and more reliable detection of the phospho: total ratio within a mixed population.

      PLC-gamma 1 is devoted to tyrosine kinase-dependent signaling for mitogenic responses to diverse agents such as growth factor activation of receptor tyrosine kinases and stimulation of immune cell receptors. Signaling pathways that depend on tyrosine kinases communicate with the PLC-gamma family. Because of its involvement in many key signaling cascades resulting from receptor tyrosine kinase activation, PLC-gamma 1 plays a critical role in tumor progression and cancer-related illnesses. When stimulating A431 cells with EGF for 2 minutes the total protein level remains constant compared to unstimulated cells, while phospho-PLC-gamma 1 levels are increased in all cells indicating that there is no competition between the two antibodies for their target epitopes.
      References
      Product Information
      Components
      • 1. 20X Anti-phospho-PLC-g1 (Tyr783) Alexa Fluor® 488: (Part No. CS206377) One vial containing 150 µL.
      • 2. 20X Anti-PLC-g1-PE: (Part No. CS206378) One vial containing 150 µL.
      • 3. Fixation Buffer: (Part No. CS202122) One bottle containing 13 mL.
      • 4. 10X Wash Buffer: (Part No. CS202123) One bottle containing 13 mL.
      • 5. 5X Assay Buffer: (Part No. CS202124) One 55 mL bottle
      • 6. 1X Permeabilization Buffer: (Part No. CS203284) One bottle containing 13 mL.
      Detection methodFluorescent
      Applications
      ApplicationThis FlowCellect PLC-g1 Activation Dual Detection Kit for Flow Cytometry includes 2 conjugated antibodies, a phospho-specific Anti-phospho-PLC-g1 (Tyr837)-Alexa Fluor 488 & an Anti-PLC-g1-PE conjugated antibody to measure total levels of PLC-g1.
      Key Applications
      • Flow Cytometry
      Biological Information
      HostMouse
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceLot to lot in-process testing on all kit components
      Usage Statement
      • Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions4-8°C for antibodies and buffers
      Packaging Information
      Material Size25 Tests / Kit
      Transport Information
      Supplemental Information
      Specifications
      Global Trade ITEM Number
      Bestellnummer GTIN
      FCCS025145 04053252748226

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