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AB5150-50UL Anti-Calcium Channel Antibody, Voltage Gated Pan α 1, CT

Anti-Calcium Channel Antibody, Voltage Gated Pan α 1, C-terminus detects level of Calcium Channel & has been published & validated for use in IC, IP & WB.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Ion Channels & Transporters, Signaling Neuroscience Gene Symbol: CACNA1C, TS, MGC120730, CACH2, Cav1.2, CaV1.2, CACNL1A1, CACN2, CCHL1A1 UniProt Number: Q13936
AB5150-50UL
50 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, RICC, IP, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB5150-50UL
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Calcium Channel Antibody, Voltage Gated Pan α 1, CT
      References
      Product Information
      FormatAffinity Purified
      PresentationAffinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 5% sucrose as a stabilizer and 0.025% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
      Quality SegmentMQ100
      Applications
      ApplicationAnti-Calcium Channel Antibody, Voltage Gated Pan α 1, C-terminus detects level of Calcium Channel & has been published & validated for use in IC, IP & WB.
      Key Applications
      • Immunocytochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesIn preparing full-length protein samples for Western blot or immunoprecipitation, the procedures should be performed at 4°C, with the following protease inhibitor mixture: pepstatin A (1 μg/mL), leupeptin (1 μg/mL), aprotinin (1 μg/mL), Pefabloc SC (0.2 mM), benzamidine (0.1 mg/mL), and calpain inhibitors I and II (8 μg/mL each) (Westenbroek et al. 1992).

      Western blot: 1:200 using ECL on rat brain membranes.

      Immunohistochemistry using rat heart sections. Note: the epitope can be masked by other subunits in some Ca2+ channels.

      Dilutions should be made using a carrier protein such as BSA (1-3%)

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenPurified peptide from {C-DNFDYLTRDWSILGPHHLD [CP15], cooresponding to residues 1506-1524 of rat alpha-1C {Accession P2202} subunit of skeletal muscle voltage-gated calcium channel (Striessnig et al. 1990), conjugated to KLH (Harlow & Lane 1988).
      EpitopeC-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes all types of alpha1 subunits of voltage-gated calcium channel. Does not cross react with any other calcium channel antigens tested so far. The epitope recongnized by the AB5150 is masked by alpha2 and delta chains, so this antibody can bind only after using Triton X 100 or other detergents that cause subunit dissociation (Ahlijanian et al. 1991).
      Species Reactivity
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes an alpha-1 subunit of a voltage-dependent calcium channel. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization. The alpha-1 subunit consists of 24 transmembrane segments and forms the pore through which ions pass into the cell. The calcium channel consists of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. There are multiple isoforms of each of these proteins, either encoded by different genes or the result of alternative splicing of transcripts. The protein encoded by this gene binds to and is inhibited by dihydropyridine. Many alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.
      Gene Symbol
      • CACNA1C
      • TS
      • MGC120730
      • CACH2
      • Cav1.2
      • CaV1.2
      • CACNL1A1
      • CACN2
      • CCHL1A1
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q13936 # Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1C gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the high-voltage activated (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to omega-conotoxin- GVIA (omega-CTx-GVIA) and omega-agatoxin-IVA (omega-Aga-IVA). Calcium channels containing the alpha-1C subunit play an important role in excitation-contraction coupling in the heart. The various isoforms display marked differences in the sensitivity to DHP compounds.
      SIZE: 2221 amino acids; 248887 Da
      SUBUNIT: Voltage-dependent calcium channels are multisubunit complexes, consisting of alpha-1, alpha-2, beta and delta subunits in a 1:1:1:1 ratio. The channel activity is directed by the pore- forming and voltage-sensitive alpha-1 subunit. In many cases, this subunit is sufficient to generate voltage-sensitive calcium channel activity. The auxiliary subunits beta and alpha-2/delta linked by a disulfide bridge regulate the channel activity. Interacts with CACNA2D4.
      SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
      TISSUE SPECIFICITY: Expressed in brain, heart, jejunum, ovary, pancreatic beta-cells and vascular smooth muscle. Overall expression is reduced in atherosclerotic vascular smooth muscle.
      DOMAIN: SwissProt: Q13936 Each of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position. & Binding of intracellular calcium through the EF-hand motif inhibits the opening of the channel (By similarity).
      PTM: Phosphorylation by PKA activates the channel (By similarity).
      DISEASE: SwissProt: Q13936 # Defects in CACNA1C are the cause of Timothy syndrome (TS) [MIM:601005]. TS is a disorder characterized by multiorgan dysfunction including lethal arrhythmias, webbing of fingers and toes, congenital heart disease, immune deficiency, intermittent hypoglycemia, cognitive abnormalities and autism.
      SIMILARITY: Belongs to the calcium channel alpha-1 subunit family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain lyophilized material at -20°C for up to 12 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size50 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade ITEM Number
      Catalogue Number GTIN
      AB5150-50UL 04053252740039

      Documentation

      Anti-Calcium Channel Antibody, Voltage Gated Pan α 1, CT SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Calcium Channel Antibody, Voltage Gated Pan α 1, CT Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-PAN α1 VOLTAGE GATED CALCIUM CHANNEL - 3231978 3231978
      RABBIT ANTI-PAN α1 VOLTAGE GATED CALCIUM CHANNEL AFFINITY PURIFIED POLYCLONAL ANTIBODY 3052517
      RABBIT ANTI-PAN α1 VOLTAGE GATED CALCIUM CHANNEL AFFINITY PURIFIED POLYCLONAL ANTIBODY 2995964
      RABBIT ANTI-PAN α1 VOLTAGE GATED CALCIUM CHANNEL AFFINITY PURIFIED POLYCLONAL ANTIBODY 3025909

      References

      Reference overviewPub Med ID
      Immunochemical indentification and subcellular distribution of the alpha 1A subunits of brain calcium channels.
      Westenbroek, R.E. et al.
      J. Neurosci., 15:6403-6418 (1995)  1995

      		7472404 7472404

      Data Sheet

      Title
      RABBIT ANTI-PAN 1 VOLTAGE GATED CALCIUM CHANNEL AFFINITY PURIFIED POLYCLONAL ANTIBODY

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies