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AB6010 Anti-MMP-12 Antibody, NT (active)

Anti-MMP-12 Antibody, N-term (active) is an antibody against MMP-12 for use in WB.

Anti-MMP-12 Antibody, NT (active) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: MMPs & TIMPs Gene Symbol: MMP12, MMP-12, MME, ME, HME UniProt Number: P39900
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AB6010
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB6010
      ReplacesAB19052
      DescriptionAnti-MMP-12 Antibody, NT (active)
      Alternate Names
      • Macrophage elastase
      • Matrix metalloproteinase-12
      • macrophage metalloelastase
      • matrix metallopeptidase 12
      Background InformationMatrix metallopeptidase 12 (MMP-12) is able to degrade elastin, entactin, laminin-1, fibronectin, type IV collagen as well as insulin B-chain and casein. MMP-12 is structurally similar to the classical MMPs (MMP-1, MMP-3); it contains a propeptide with autoinhibitory cysteine switch site, a well conserved zinc site, hinge region and hemopexin domain. MMP-12 lacks a transmembrane domain and furin cleavage site. The zymogen for MMP-12 is about 54 kDa, and is quickly activated to the 45 kDa form, and this breaks down to cascade of active forms, ending with the common 22 kDa form.Stimulated macrophages produce MMP-12; it has also been found in osteosarcoma cells, synovial fibroblasts and lung fibroblasts. MMP-12 levels in quiescent cells and tissues are minimal (with the exception of macrophages), and mitogen stimulation or protein concentration is often needed to visualize the bands. In addition, cell types differ greatly in the quantity of MMP-12 produced.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Concentrated conditioned cell culture media collected from a proprietary human fibroblast cell line.
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Quality SegmentMQ100
      Applications
      ApplicationAnti-MMP-12 Antibody, N-term (active) is an antibody against MMP-12 for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated synthetic peptide corresponding to human MMP-12 near the N-terminus.
      EpitopeN-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes MMP-12 near the N-terminus of the active form.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Sequence homology to mouse is 84%.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryProteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. It is thought that the protein encoded by this gene is cleaved at both ends to yield the active enzyme, but this processing has not been fully described. The enzyme degrades soluble and insoluble elastin. It may play a role in aneurysm formation and studies in mice suggest a role in the development of emphysema. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3.
      Gene Symbol
      • MMP12
      • MMP-12
      • MME
      • ME
      • HME
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3.

      COFACTOR: Binds 4 calcium ions per subunit. & Binds 2 zinc ions per subunit.

      SIZE: 470 amino acids; 54002 Da

      TISSUE SPECIFICITY: Found in alveolar macrophages but not in peripheral blood monocytes.

      DOMAIN: The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

      SIMILARITY: Belongs to the peptidase M10A family. & Contains 4 hemopexin-like domains.
      Molecular WeightThe zymogen for MMP-12 is ~ 54 kDa, and is quickly activated to a 45 kDa form. This breaks down to a cascade of active forms, ending with the common 22 kDa form.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot using a concentrated conditioned cell culture media collected from a proprietary human fibroblast cell line (Cat. No. AG902) and probing with 1 µg/mL of Anti-MMP-12.

      Western Blot Analysis: 1 µg/ml of this antibody detected MMP-12 on 10 µg of lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade ITEM Number
      Catalogue Number GTIN
      AB6010 04053252740473

      Documentation

      Anti-MMP-12 Antibody, NT (active) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-MMP-12 Antibody, NT (active) Certificates of Analysis

      TitleLot Number
      Anti-MMP-12, N-term (active) - 2436369 2436369
      Anti-MMP-12, N-term (active) - 2033660 2033660
      Anti-MMP-12, N-term (active) - 2829138 2829138
      Anti-MMP-12, N-term (active) - 3826052 3826052
      Anti-MMP-12, N-term (active) - 4006706 4006706
      Anti-MMP-12, N-term (active) - NG1740764 NG1740764
      Anti-MMP-12, N-term (active) - NG1867626 NG1867626
      Anti-MMP-12, N-term (active) - NRG1671160 NRG1671160
      Anti-MMP-12, N-term (active) -2743625 2743625
      Anti-MMP-12, N-term (active) -2853589 2853589
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