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SCC449 OSUMMER.5 Mouse NRAS-Mutant Melanoma Cell Line

OSUMMER.5 male mouse NRAS-mutant melanoma cell line carries the TN61R mutation and is derived from the TN mouse model. This model is syngeneic with the C57BL/6 mouse model. OSUMMER cells can form tumors in vivo without Matrigel injection.

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SCC449
≥1x10˄6 cells/vial  
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      Overview

      Replacement Information
      Description
      Catalogue NumberSCC449
      DescriptionOSUMMER.5 Mouse NRAS-Mutant Melanoma Cell Line
      Alternate Names
      • Mouse melanoma cell line
      Background InformationMelanoma is a skin cancer that develops in pigment-producing melanocytes. Sporadic genetic mutations have been associated with melanomas. Immunotherapy treatments targeting these genes or pathways have been proven to have significant benefits in melanoma patients by improving general response and survival.

      The OSUMMER (Ohio State University and Moffitt Melanoma Exposed to Radiation)cell lines fulfill a previously unfilled gap in melanoma biology. They are derived from the TN mouse model of melanoma, which are homozygous for the LSL-Nras Q61R allele. Oncogene expression is activated by tamoxifen-inducible Cre recombinase in melanocytes.
      Loss of function is an early event observed in the majority of human melanomas. TN mice additionally carry p16INK4A conditional knockout alleles.

      OSUMMER.5 NRAS mouse melanoma cell line was derived from a male TN mouse exposed to UVA and UVB radiation. OSUMMER.5 carries the specific NRAS mutation Q61R.WES revealed high SNV (Single Nucleotide variants) and low CNA (Copy Number Alterations) burden, as well as the presence of UV signature mutations. OSUMMER.5 cells express the melanoma markers PMEL and MelanA, and exhibit robust growth in culture.

      Source
      OSUMMER.5 was derived from melanoma induced in TN (Tyr::CreER) mice exposed to UVA or UVB radiation on postnatal day 3.

      References
      1. Nat Rev Dis Primers 2015, 1: 15003
      2.The Lancet, 392(10151), 971-984.
      3. Life Sci Alliance 2021, 4(9): e202101135.
      References
      Product Information
      Quality SegmentMQ100
      Applications
      ApplicationOSUMMER.5 male mouse NRAS-mutant melanoma cell line carries the TN61R mutation and is derived from the TN mouse model. This model is syngeneic with the C57BL/6 mouse model. OSUMMER cells can form tumors in vivo without Matrigel injection.
      Key Applications
      • Cell Culture
      • Cell Growth
      • Pharmaceutical
      Application NotesThis product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
      Biological Information
      Cell Line Type
      • Cancer Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEach vial contains ≥1X10⁶ viable cells.

      Cells are tested negative for infectious diseases by a Mouse Essential CLEAR Panel and have been found to be negative for all pathologies in this test.

      Cells are verified to be of mouse origin and negative for interspecies contamination from rat, human, Chinese hamster, Golden Syrian hamster, and non-human primate (NHP) as assessed by a Contamination Clear panel.

      Cells are negative for mycoplasma contamination.

      Cells are genotyped by STR analysis to verify the unique identity of the cell line.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      Storage and Shipping Information
      Storage ConditionsOSUMMER.5 cells should be stored in liquid nitrogen until use. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
      Packaging Information
      Material Size≥1x10˄6 cells/vial
      Transport Information
      Supplemental Information
      Specifications
      Global Trade ITEM Number
      Catalogue Number GTIN
      SCC449 04065271213920

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