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PC386 PhosphoDetect™ Anti-p53 (pSer¹⁵) (Ab-3) Rabbit pAb

This PhosphoDetect™ Anti-p53 (pSer¹⁵) (Ab-3) Rabbit pAb is validated for use in Immunoblotting , Immunoprecipitation for the detection of p53 (pSer¹⁵) (Ab-3).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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PC386
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, M, RRbPolyclonal Antibody

Pricing & Availability

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PC386-50UL
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      Description
      OverviewRecognizes the ~53 kDa p53 protein phosphorylated at Ser15 in PC12 or HeLa cells treated with DNA damaging agents. Does not recognize unphosphorylated p53.
      Catalogue NumberPC386
      Brand Family Calbiochem®
      Application Data
      Detection of human p53, phophorylated on Ser15, by immunoblotting. Samples: Whole cell lysates from untreated HCT116 cells (Lane A) and HCT116 cells treated with ionizing radiation (Lane B). Primary antibody: PhosphoDetect™ Anti-p53 (pSer15) (Ab-3) Rabbit pAb (Cat. No. PC386) (1:1000). Detection: chemiluminescence.
      References
      ReferencesTibbetts, R.S., et al. 1999. Genes Dev. 13, 152.
      Levine, A.J. 1997. Cell 88, 323.
      Milczarek, G.J., et al. 1997. Life Sci. 60, 1.
      Shieh, S.Y., et al. 1997. Cell 91, 325.
      Meek, D.W. 1994. Semin Cancer Biol. 5, 203.
      Product Information
      FormLiquid
      FormulationIn 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.
      Positive controlPC-12, HCT116, or HeLa cells treated with DNA damaging agents
      PreservativeNone
      Quality SegmentMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunoprecipitation
      Application NotesImmunoblotting (1:1000)
      Immunoprecipitation (1:200)
      Application CommentsDoes not recognize non-phosphorylated p53. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser¹⁵ phosphorylation site of human p53, conjugated to KLH
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C). Avoid freeze/thaw cycles.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade ITEM Number
      Catalogue Number GTIN
      PC386-50UL 04055977224559

      Documentation

      PhosphoDetect™ Anti-p53 (pSer¹⁵) (Ab-3) Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      PhosphoDetect™ Anti-p53 (pSer¹⁵) (Ab-3) Rabbit pAb Certificates of Analysis

      TitleLot Number
      PC386

      References

      Reference overview
      Tibbetts, R.S., et al. 1999. Genes Dev. 13, 152.
      Levine, A.J. 1997. Cell 88, 323.
      Milczarek, G.J., et al. 1997. Life Sci. 60, 1.
      Shieh, S.Y., et al. 1997. Cell 91, 325.
      Meek, D.W. 1994. Semin Cancer Biol. 5, 203.

      Brochure

      Title
      Caspases and other Apoptosis Related Tools Brochure
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision28-April-2008 JSW
      ApplicationImmunoblotting (1:1000)
      Immunoprecipitation (1:200)
      Application Data
      Detection of human p53, phophorylated on Ser15, by immunoblotting. Samples: Whole cell lysates from untreated HCT116 cells (Lane A) and HCT116 cells treated with ionizing radiation (Lane B). Primary antibody: PhosphoDetect™ Anti-p53 (pSer15) (Ab-3) Rabbit pAb (Cat. No. PC386) (1:1000). Detection: chemiluminescence.
      DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~53 kDa p53 protein phosphorylated on Ser15.
      Backgroundp53 tumor suppressor proteins play a key role in cellular defense against DNA damaging agents. p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro. DNA damage results in the phosphorylation of p53 at Ser15, leading to the reduced interactions of p53 with its negative regulator, the oncoprotein MDM2. p53 can be phosphorylated by ATM, by ATR, and by the DNA-dependent protein kinase (DNA-PK) at Ser15. This impairs the ability of MDM2 to bind p53 and promotes both the accumulation and functional activation of p53 in response to DNA damage.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser¹⁵ phosphorylation site of human p53, conjugated to KLH
      IsotypeIgG
      Specieshuman, mouse, rat
      Positive controlPC-12, HCT116, or HeLa cells treated with DNA damaging agents
      FormLiquid
      FormulationIn 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.
      PreservativeNone
      CommentsDoes not recognize non-phosphorylated p53. Antibody should be titrated for optimal results in individual systems.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C). Avoid freeze/thaw cycles.
      Toxicity Standard Handling
      ReferencesTibbetts, R.S., et al. 1999. Genes Dev. 13, 152.
      Levine, A.J. 1997. Cell 88, 323.
      Milczarek, G.J., et al. 1997. Life Sci. 60, 1.
      Shieh, S.Y., et al. 1997. Cell 91, 325.
      Meek, D.W. 1994. Semin Cancer Biol. 5, 203.

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