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07-770 Anti-acetyl-Histone H2A.Z (Lys8) Antibody | 07-770

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Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histones Gene Symbol: H2A.z, H2A/z, H2AZ, H2AFZ UniProt Number: Q12692
07-770
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      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-770
      DescriptionAnti-acetyl-Histone H2A.Z (Lys8) Antibody | 07-770
      Alternate Names
      • H2A histone family, member Z
      • H2AZ histone
      • histone H2A.Z
      • Htz1
      Background InformationH2A.Z, also known as H2AFZ, is a variant of histone H2A variant that is highly conserved from yeast to humans. Like other histone variants, H2A.Z is encoded by a unique gene, resulting in a slightly divergent type of H2A with specialized functions. H2A.Z is involved in chromatin compaction by stabilizing the histone octamer within the nucleosome. It promotes gene expression through the creation of an open domain of chromatin that is more easily transcribed. H2A.Z is nonrandomly distributed throughout the genome, targeted primarily to pericentric heterochromatin, but excluded from the inactive X chromosome and the nucleolus. H2A.Z is associated with both euchromatin and facultative heterochromatin. DNA methylation can influence chromatin structure and effect gene silencing by excluding H2A.Z, and likewise, H2A.Z protects genes from DNA methylation. Thus, H2A.Z plays a role in regulating heterochromatin silencing and is involved in transcriptional control.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Yeast whole cell extract
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      Key Applications
      • Western Blotting
      Application NotesWestern Blot (Snap i.d.) Analysis: 4 µg/mL from a representative lot detected Histone H2A.Z on 10 µg of yeast whole cell extract.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to yeast Histone H2A.Z acetylated at Lys8.
      EpitopeHistone H2A.Z acetylated Lys8
      Concentration0.5 mg/mL
      HostRabbit
      SpecificityThis antibody recognizes Histone H2A.Z when acetylated at Lys8.
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • H2A.z
      • H2A/z
      • H2AZ
      • H2AFZ
      Modifications
      • Acetylation
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Variant histone H2A which can replace H2A in some nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. This variant is enriched at promoters, it may keep them in a repressed state until the appropriate activation signal is received. Near telomeres, it may counteract gene silencing caused by the spread of heterochromatin proteins. Required for the RNA polymerase II and SPT15/TBP recruitment to the target genes. Involved in chromosome stability.

      SUBUNIT STRUCTURE: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. H2A or its variant H2A.Z forms an heterodimer with H2B. H2A.Z associates with the VPS72/SWC2 subunit of the SWR1 chromatin remodeling complex. Interacts also with RBP1/DNA-directed RNA polymerase II largest subunit. Interacts with NAP1.

      SUBCELLULAR LOCATION: Nucleus

      PTM: Acetylated by ESA1, a component of the NuA4 histone acetyltransferase (HAT) complex, and/or by GCN5, a component of the SAGA complex, to form H2A.ZK3Ac, H2A.ZK8Ac, H2A.ZK10Ac and H2A.ZK14Ac once deposited into chromatin. Acetylation is required for function at telomeres. H2A.ZK14Ac is acetylated at the promoters of active genes.

      MISCELLANEOUS: Present with 2840 molecules/cell in log phase SD medium.

      In contrast to H2A1 and H2A2, appears to be weakly or not sumoylated.

      SEQUENCE SIMILARITIES: Belongs to the histone H2A family.

      CAUTION: To ensure consistency between histone entries, we follow the 'Brno' nomenclature for histone modifications, with positions refering to those used in the literature for the 'closest' model organism. Due to slight variations in histone sequences between organisms and to the presence of initiator methionine in UniProtKB/Swiss-Prot sequences, the actual positions of modified amino acids in the sequence generally differ. In this entry the following conventions are used: H2A.ZK3ac = acetylated Lys-4; H2A.ZK8ac = acetylated Lys-9; H2A.ZK10ac = acetylated Lys-11; H2A.ZK14ac = acetylated Lys-15.
      Molecular Weight~ 16 kDa observed. Slightly cross-reacting band ~14 kDa (Histone H4) with TBS-T (01%).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in yeast whole cell extract.

      Western Blot Analysis: 0.5 µg/mL of this antibody detected Histone H2A.Z on 10 µg of yeast whole cell extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade ITEM Number
      Référence GTIN
      07-770 04053252747724

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      Catégories

      Life Science Research > Antibodies and Assays > Primary Antibodies