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UFC910096 Amicon Ultra-15, membrana PLHK Ultracel-PL, 100 kDa

Ultracel-100 regenerated cellulose membrane, 15 mL sample volume

Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Aplicaciones clave: Extracción de proteínas, Purificación de proteínas
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UFC910096
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      Descripción

      Replacement Information

      Tabla espec. clave

      Key ApplicationsLímite de peso molecular nominal (NMWL)VolumenVolumen de concentrado final mínimo
      Extracción de proteínas, Purificación de proteínas100 KDa15 mL200 µL
      Descripción
      Número de catálogoUFC910096
      Nombre comercial
      • Amicon Ultra
      DescripciónAmicon Ultra-15, membrana PLHK Ultracel-PL, 100 kDa
      Información preliminarAMICON ULTRA-15 15ML - 100 KDa cutoff. Available in 3 pack sizes.
      UFC910008 - 8 pack
      UFC910024 - 24 pack
      UFC910096 - 96 pack



      High recovery Ultracel regenerated cellulose membrane in a range of molecular weight cut-offs
      High retentate recovery of greater than 90%
      Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 10–15 minutes)
      Heat-sealed membrane minimizes downstream extractables
      100% integrity tested for reliable performance
      Convenient sample monitoring with translucent housing and volume gradations
      Direct pipettor sample access eliminates processing step to recover concentrate
      High concentration factors of 80–100X

      Applications:
      Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
      Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLS
      Concentration of dilute or pre-purified proteins from column eluents
      Desalting, buffer exchange and protein dialysis
      Referencias bibliográficas
      Información del producto
      Código HS 8421 29 90
      Código del filtroPLHK
      Código de coloresTransparente con tapa rosa
      Tipo de filtroUltrafiltración
      Límite de peso molecular nominal (NMWL)100 KDa
      Quality SegmentMQ400
      Aplicaciones
      Aplicación Ultracel-100 regenerated cellulose membrane, 15 mL sample volume
      Aplicaciones clave
      • Extracción de proteínas
      • Purificación de proteínas
      Notas de aplicaciónRotor de cabezal basculante: 4.000 x g; Rotor de ángulo fijo (35°): 5.000 g
      Información biológica
      EsterilidadNo estéril
      Información fisicoquímica
      Aplicación centrífugaProtein Concentration and Desalting
      Dimensiones
      Longitud12.1 cm (4.807 in.)
      Diámetro2.97 cm
      Área de filtración7.6 cm²
      Volumen de concentrado final mínimo200 µL
      Volumen15 mL
      Información de materiales
      Química
      • Regenerated Cellulose (RC)
      Material del dispositivo
      • Housing: Styrene/butadiene
      • Tubo: Polipropileno
      • Tapa: Polietileno
      Material de soporteCelulosa regenerada
      Información toxicológica
      Información de seguridad según el GHS
      Información de seguridad
      Declaraciones de uso del producto
      Información de almacenamiento y transporte
      Información sobre embalaje
      Cantitad96
      Información de transporte
      Información complementaria
      Especificaciones
      Global Trade ITEM Number
      Número de referencia GTIN
      UFC910096 04053252316517

      Documentation

      Folleto

      Cargo
      REFINE PROTEIN PREPARATION. Tools for better protein analysis

      Preguntas frecuentes

      PreguntaRespuesta
      Can Amicon Ultra units be sterilized with ethanol?Amicon Ultra is compatible with 70% Ethanol, but we have not done any studies to test for sterilization.
      Do you have any centrifugal filter units that are pyrogen free?We do not have any centrifugals devices that we claim are pyrogen free. Even the sterile devices could contain pyrogens.
      What pipette or pipette tips do you recommend to recover the retentate from the Amicon Ultra concentrators?We do not have any recommendation for pipette or pipette tip in the insert-booklet of Amicon Ultra. Certain pipettes, like Eppendorf 100-1000µl, are very wide and the tip can not reach the bottom of the device. So a smaller pipette is needed. The pipette we use for all the studies is a Gilson pipetman with regular pipette tips 1-200µl from Fisher cat# 21-197-2J.
      Amicon Ultra units are offered in what volumes?Amicon Ultra units are available in 0.5 mL, 4mL & 15ml volumes.
      What is the recommended rotor for Amicon Ultra units?Any rotor capable of accommodating 22.7 cm in diameter and 124 mm length centrifuge tubes for the Amicon Ultra-4 and 35 mm in diameter and 121 mm in length for the Amicon Ultra-15 can be used for Amicon Ultra units.
      How should I select the appropriate Molecular Weight Cutoff (MWCO) for Amicon Ultra?Select a Molecular Weight Cutoff that is 2x smaller that the Molecular Weight (MW) of the solute of interest.
      Which membranes are available in Amicon Ultra units?Amicon Ultra units are only available with Ultracel regenerated cellulose material. This high recovery/low binding membrane is offered in 3,000, 10,000, 30,000, 50,000 & 100,000 MWCO.
      Can the Amicon Ultra be spun to dryness?No, the Amicon Ultra has a built in deadstop.
      Can the Amicon Ultra be used with nucleic acids?Yes, Amicon Ultra units have been evaluated for use with Genomic and PCR fragments. The 30 kDa MWCO provides the best balance between recovery and spin time. Protocols related to Genomic and PCR fragments can be found in the user guide.
      What is the maximum sample volume for Amicon Ultra?
      Amicon Ultra-4Amicon Ultra-15
      Swinging Bucket4ml 15ml
      Fixed Angle4ml(35 & 45 degree rotor angle)12ml(35 degree rotor angle)
      3.5ml(23 degree rotor angle)
      Show more

      Manuales del usuario

      Cargo
      Amicon® Ultra-15 Centrifugal Filter Devices Non IVD User guide