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ST1088 Anti-Akt, PH Domain Mouse mAb (SKB1)

Anti-Akt, PH Domain, mouse monoclonal, clone SKB1, recognizes the ~60 kDa Akt protein in A431 cells. It is validated for flow cytometry, Western blotting, and immunoprecipitation.

Anti-Akt, PH Domain Mouse mAb (SKB1) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-PKB, Pleckstrin Homology Domain

ST1088
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, M, RMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
ST1088-1SETCN Plastic ampoule 1 set
Description
OverviewRecognizes the ~60 kDa Akt protein in A431 cells. Useful for the concentration of Akt in samples by immunoprecipitation prior to use in the K-LISA™ Akt Activity Kit (Cat. No. CBA019).
  • Antibody Target Gene Symbol: AKT1
  • Target Synonym: AKT, MGC99656, PKB, PKB-ALPHA, PKB/AKT, PRKBA, Protein kinase B, RAC, RAC-ALPHA, Thymoma viral proto-oncogene 1
  • Entrez Gene Name: v-akt murine thymoma viral oncogene homolog 1
  • Hu Entrez ID: 207 (Related Antibodies: 530311, PK1203, PC510 and 124001)
  • Mu Entrez ID: 11651
  • Rat Entrez ID: 24185
    		•
    Catalogue NumberST1088
    Brand Family Calbiochem®
    SynonymsAnti-PKB, Pleckstrin Homology Domain
    Application Data
    Detection of human Akt by immunoblotting. Sample: Whole cell lysate from A431 cells. Primary antibody: Anti-Akt, PH Domain Mouse mAb (SKB1) (Cat. No. ST1088) (0.5 µg/ml). Detection: chemiluminescence.
    References
    ReferencesBellacosa, A., et al. 1998. Oncogene 17, 313.
    Product Information
    FormLiquid
    Formulation100 µg antibody in 150 mM NaCl, 100 mM Tris-glycine, 30% glycerol, pH 7.4 and 100 µg control lysate.
    Positive controlA431 cells (included)
    Preservative≤0.1% sodium azide
    Quality SegmentMQ100
    Applications
    Application ReferencesImmunoblotting Bourguignon, L.Y.W., et al. 2003. J. Biol. Chem. 278, 29420. Beffert, U., et al. 2002. J. Biol. Chem. 277, 49958. Immunoprecipitation, Active Enzyme Beffert, U., et al. 2002. J. Biol. Chem. 277, 49958.
    Key Applications Flow Cytometry
    Immunoblotting (Western Blotting)
    Immunoprecipitation
    Application NotesFlow Cytometry (see comments)
    Immunoblotting (0.5-2 µg/ml, see application references)
    Immunoprecipitation (4 µg/0.5 mg total protein; see comments and see application references)
    Application CommentsThis antibody will immunoprecipitate active Akt from cell lysates. Useful for immunoprecipitation of Akt prior to activity assay using the K-LISA™ Akt Activity Kit (Cat. No. CBA019). This antibody has also been reported to work for flow cytometry applications. Antibody should be titrated for optimal results in individual systems.
    Biological Information
    Immunogena recombinant protein consisting of amino acids 1-149 of human Akt 1 fused to GST
    ImmunogenHuman
    CloneSKB1
    HostMouse
    IsotypeIgG
    Species Reactivity
    • Human
    • Mouse
    • Rat
    Antibody TypeMonoclonal Antibody
    Concentration Label Please refer to vial label for lot-specific concentration
    Physicochemical Information
    Dimensions
    Materials Information
    Toxicological Information
    Safety Information according to GHS
    Safety Information
    Product Usage Statements
    Storage and Shipping Information
    Ship Code Shipped with Blue Ice or with Dry Ice
    Toxicity Standard Handling
    Storage -20°C
    Avoid freeze/thaw Avoid freeze/thaw
    Do not freeze Ok to freeze
    Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
    Packaging Information
    Transport Information
    Supplemental Information
    Specifications
    Global Trade ITEM Number
    Catalogue Number GTIN
    ST1088-1SETCN 04055977224412

    Documentation

    Anti-Akt, PH Domain Mouse mAb (SKB1) Certificates of Analysis

    TitleLot Number
    ST1088

    References

    Reference overview
    Bellacosa, A., et al. 1998. Oncogene 17, 313.

    Brochure

    Title
    Akt
    Biologics 31.1
    Diabetes and Obesity
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision04-August-2008 JSW
    SynonymsAnti-PKB, Pleckstrin Homology Domain
    ApplicationFlow Cytometry (see comments)
    Immunoblotting (0.5-2 µg/ml, see application references)
    Immunoprecipitation (4 µg/0.5 mg total protein; see comments and see application references)
    Application Data
    Detection of human Akt by immunoblotting. Sample: Whole cell lysate from A431 cells. Primary antibody: Anti-Akt, PH Domain Mouse mAb (SKB1) (Cat. No. ST1088) (0.5 µg/ml). Detection: chemiluminescence.
    DescriptionProtein G purified mouse monoclonal antibody. Recognizes ~50-60 kDa Akt1 and Akt2 proteins. Supplied with control lysate derived from unstimulated A431 cells.
    BackgroundAkt (Accession No. NM_005163) is primarily a cytosolic enzyme that acts as a downstream effector of phosphatidylinositol 3-kinase (PI-3K) activation. Akt has been shown to be activated by a variety of stimuli, including hematopoetic cytokines, various chemokines, heat shock, hypoxia,hyperosmolarity, integrins, nerve growth factor (NGF), platelet- derived growth factor (PDGF), and T cell receptor signaling. Suggested to play a role in regulating the balance between cellular mitogenesis and apoptosis. Akt has been shown to be constitutively active in some human cancers.
    HostMouse
    Immunogen speciesHuman
    Immunogena recombinant protein consisting of amino acids 1-149 of human Akt 1 fused to GST
    CloneSKB1
    IsotypeIgG
    Specieshuman, mouse, rat
    Positive controlA431 cells (included)
    FormLiquid
    Formulation100 µg antibody in 150 mM NaCl, 100 mM Tris-glycine, 30% glycerol, pH 7.4 and 100 µg control lysate.
    Concentration Label Please refer to vial label for lot-specific concentration
    Preservative≤0.1% sodium azide
    CommentsThis antibody will immunoprecipitate active Akt from cell lysates. Useful for immunoprecipitation of Akt prior to activity assay using the K-LISA™ Akt Activity Kit (Cat. No. CBA019). This antibody has also been reported to work for flow cytometry applications. Antibody should be titrated for optimal results in individual systems.
    Storage Avoid freeze/thaw
    -20°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
    Toxicity Standard Handling
    ReferencesBellacosa, A., et al. 1998. Oncogene 17, 313.
    Application referencesImmunoblotting Bourguignon, L.Y.W., et al. 2003. J. Biol. Chem. 278, 29420. Beffert, U., et al. 2002. J. Biol. Chem. 277, 49958. Immunoprecipitation, Active Enzyme Beffert, U., et al. 2002. J. Biol. Chem. 277, 49958.