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MABE1052 Anti-GMPS Antibody, clone 1G6.1

Anti-GMPS, clone 1G6.1, Cat. No. MABE1052, is a highly specific mouse monoclonal antibody that targets GMPS and has been tested in Western Blotting.

Anti-GMPS Antibody, clone 1G6.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: GMPS UniProt Number: P49915
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MABE1052
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE1052
DescriptionAnti-GMPS Antibody, clone 1G6.1
Alternate Names
  • GMP synthase [glutamine-hydrolyzing]
  • Glutamine amidotransferase
  • GMP synthetase
Background InformationGMP synthase [glutamine-hydrolyzing] (EC 6.3.5.2; UniProt P49915; also known as Glutamine amidotransferase, GMP synthetase) is encoded by the GMPS gene (Gene ID 8833) in human. De novo biosynthesis of Guanosine 5'-monophosphate (GMP) is accomplished by reactions catalyzed by several enzymes. Inositol monophosphate (IMP) dehydrogenase 1 and 2 (IMPDH1 and IMPDH2) convert IMP into xanthosine monophosphate (XMP), GMP synthetase (GMPS) then converts XMP into GMP, while guanosine monophosphate reductase (GMPR) catalyzes the reversible conversion of GMP to IMP to fuel back into both the AMP and GMP synthesis pathways. GMPS is a bifunctional two-domain N-type pyrophosphatase whose N-terminal glutaminase domain (a.a. 27-216) generates ammonia from glutamine, while its C-terminal synthetase domain (a.a. 27-435) adenylylates XMP on the xanthine C2 oxygen, thereby priming XMP for attack by a nitrogen nucleophile to form GMP. Human GMPS activity is subjected to an allosteric regulation and displays positive cooperativity for the substrate XMP. GMPS is normally sequestered in the cytoplasm, but translocates to the nucleus in response to genotoxic stress or nucleotide deprivation. GMPS nuclear translocation plays an important role in USP7-mediated stabilization of p53, where p53 is transferred from MDM2 to the GMPS-USP7 deubiquitylation complex.
References
Product Information
FormatPurified
PresentationPurified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality SegmentMQ100
Applications
ApplicationAnti-GMPS, clone 1G6.1, Cat. No. MABE1052, is a highly specific mouse monoclonal antibody that targets GMPS and has been tested in Western Blotting.
Key Applications
  • Western Blotting
Biological Information
ImmunogenGST-tagged recombinant human GMPS ATP-PPase domain fragment.
Clone1G6.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 1G6.1 targets an epitope within the GMPS ATP-PPase domain region present in both spliced isoforms of human GMPS reported by UniProt (P49915).
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • GMPS
Purification MethodProtein G purified
UniProt Number
Molecular Weight~70 kDa observed. 76.58/65.80 kDa (isoform 1/2) calculated (Met1 removed). Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected GMPS in 10 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade ITEM Number
Catalogue Number GTIN
MABE1052 04054839090240