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ABT168 Anti-Girdin Antibody

This Girdin antibody is validated for use in WB, IHC, IF & IP for the detection of the Girdin protein.

Anti-Girdin Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Cytoskeleton
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ABT168
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, Mk, RWB, IH(P), IF, IPRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABT168
DescriptionAnti-Girdin Antibody
Background InformationGirdin (Ccdc88a; APE, GIV, or HkRP1) is a ubiquitous protein belonging to the CCDC88 family. Girdin is an actin-binding protein which interacts with actin via its C-terminal domain, and may play a crucial role in cell motility and cytoskeleton remodeling. These effects may be regulated by a reciprocal relationship between Girdin and Akt. Akt phosphorylates Girdin, and Girdin binds to Akt and enhances phosphorylation of Akt, thereby promoting phosphorylation of Akt’s downstream targets such as GSK-3β and FOXO1A. Girdin/Akt signaling may play an important role in cancer by facilitating the proliferation of cancer cells.
References
Product Information
FormatAffinity Purified
Control
  • Cos-7 cell lysate
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality SegmentMQ100
Applications
ApplicationThis Girdin antibody is validated for use in WB, IHC, IF & IP for the detection of the Girdin protein.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
  • Immunofluorescence
  • Immunoprecipitation
Application NotesImmunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected Girdin in human colorectal cancer tissues.

Immunofluorescence Analysis: A 1:500 dilution from a representative lot detected Girdin in HeLa, Cos-7, and Att20 cells.

Immunoprecipitation Analysis: A representative lot immunoprecipitated Girdin in HeLa cell lysate.
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to the coiled coil domain rat Girdin.
EpitopeCoiled coil domain.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis antibody recognizes the coiled-coil domain of Girdin.
Species Reactivity
  • Human
  • Monkey
  • Rat
Species Reactivity NoteDemonstrated to react with Human and Monkey. Predicted to react with Rat based upon immunogen design. Other homologies: Mouse (97% sequence homology).
Antibody TypePolyclonal Antibody
Entrez Gene Number
Purification MethodAffinity Purfied
Molecular Weight~220 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Cos-7 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Girdin in 10 µg of Cos-7 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade ITEM Number
Catalogue Number GTIN
ABT168 04053252858277