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MABS810 Anti-MAP2K6, clone 3H12C8 Antibody

Anti-MAP2K6, clone 3H12C8 Antibody is an antibody against MAP2K6 for use in Western Blotting, Immunocytochemistry, Flow Cytometry, ELISA.

Anti-MAP2K6, clone 3H12C8 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Developmental Signaling Gene Symbol: MAP2K6, MEK6, MKK6, PRKMK6, SKK3 UniProt Number: P52564
MABS810
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, ICC, FC, ELISAMAscitesMonoclonal Antibody
Description
Catalogue NumberMABS810
Replaces04-383
DescriptionAnti-MAP2K6, clone 3H12C8 Antibody
Alternate Names
  • Dual specificity mitogen-activated protein kinase kinase 6
  • MAP kinase kinase 6
  • MAPKK 6
  • MAP2K6
Background InformationDual specificity mitogen-activated protein kinase kinase 6 (MP2K6) is a member of the dual specificity protein kinase family, which functions as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. MP2K6 phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines or environmental stress. As an essential component of p38 MAP kinase mediated signal transduction pathway, this gene is involved in many cellular processes such as stress induced cell cycle arrest, transcription activation and apoptosis.
References
Product Information
FormatAscites
PresentationMouse monoclonal IgG1ascitic fluid containing 0.03% sodium azide.
Quality SegmentMQ300
Applications
ApplicationAnti-MAP2K6, clone 3H12C8 Antibody is an antibody against MAP2K6 for use in Western Blotting, Immunocytochemistry, Flow Cytometry, ELISA.
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Flow Cytometry
  • ELISA
Application NotesImmunocytochemistry Analysis (ICC): A 1:200-1,000 dilution from a representative lot detected MAP2K6 in HeLa cells.

Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected MAP2K6 in HeLa cells.

ELISA: A representative lot was used on control antigen (100 ng) and antigen (10 ng, 50 ng, 100 ng) in ELISA (Direct).
Biological Information
ImmunogenPurified recombinant fragment of human MAP2K6 expressed in E. Coli.
Clone3H12C8
ConcentrationPlease refer to lot specific datasheet.
HostMouse
IsotypeIgG1
Species Reactivity
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Gene Symbol
  • MAP2K6
  • MEK6
  • MKK6
  • PRKMK6
  • SKK3
Purification MethodUnpurified
UniProt Number
Molecular Weight~38 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HepG2, MCF-7 and NIH/3T3 cell lysates.

Western Blotting Analysis (WB): A 1:500-2,000 dilution of this antibody detected MAP2K6 in HepG2, MCF-7 and NIH/3T3 cell lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade ITEM Number
Catalogue Number GTIN
MABS810 04055977172218

Documentation

Anti-MAP2K6, clone 3H12C8 Antibody Certificates of Analysis

TitleLot Number
Anti-MAP2K6, clone 3H12C8 -QVP1502050 QVP1502050
Anti-MAP2K6, clone 3H12C8 -VP1609042 VP1609042
Anti-MAP2K6, clone 3H12C8 Monoclonal Antibody VP1704270
Anti-MAP2K6, clone 3H12C8 Monoclonal Antibody VP1801124

Technical Info

Title
Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
White Paper: Further considerations of antibody validation and usage.