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MABS1250 Anti-Mineralocorticoid Receptor Antibody, clone 2D6

Detect Mineralocorticoid Receptor using this Anti-Mineralocorticoid Receptor Antibody, clone 2D6 validated for use in Western Blotting, Immunohistochemistry and Immunocytochemistry.

Anti-Mineralocorticoid Receptor Antibody, clone 2D6 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Signaling Neuroscience Gene Symbol: Nr3c2, Mlr UniProt Number: P22199
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MABS1250
200 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
RWB, IHC, ICCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS1250
DescriptionAnti-Mineralocorticoid Receptor Antibody, clone 2D6
Alternate Names
  • MR
  • Nuclear receptor subfamily 3 group C member 2
  • Mineralocorticoid Receptor
Background InformationMineralocorticoid receptor (UniProt P22199; also known as MR, Nuclear receptor subfamily 3 group C member 2) is encoded by the Nr3c2 (also known as MCR, Mlr) gene (Gene ID 25672) in rats. Mineralocorticoid receptor (MR) is expressed in multiple tissues, including renal epithelia, smooth muscle, endothelium, cardiomyocytes, and hippocampal neurons, where it mediates diverse functions. MR is activated by aldosterone and also by cortisol in cells that do not express 11β-hydroxysteroid dehydrogenase type 2 (11βHSD2). MR is normally activated by aldosterone, which is produced by adrenal glomerulosa in response to intravascular volume depletion and hyperkalemia. Consistently, gain of function mutations in MR lead to severe hypertension, often with hypokalemia, while loss of function mutations result in neonatal hypotension. Ser843 phosphorylation in the MR ligand-binding domain is reported to prevent MR ligand binding and activation. MR pS843 is found exclusively in intercalated cells of the distal nephrons in the kidney. Intravascular volume depletion, angiotensin II and WNK4 signaling reduce MR pS843 levels, whereas hyperkalemia increases MR pS843.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality SegmentMQ100
Applications
ApplicationDetect Mineralocorticoid Receptor using this Anti-Mineralocorticoid Receptor Antibody, clone 2D6 validated for use in Western Blotting, Immunohistochemistry and Immunocytochemistry.
Key Applications
  • Western Blotting
  • Immunohistochemistry
  • Immunocytochemistry
Application NotesImmunohistochemistry Analysis: A representative lot detected Mineralocorticoid Receptor in hippocampus, dentate gyrate, choroid plexus, heart, and rat kidney (Gomez-Sanchez, C.E., et al. (2006). Endocrinology. 147(3):1343-1348).
Immunocytochemistry Analysis: A representative lot detected Mineralocorticoid Receptor in mouse kidney (Shibata, S., et al. (2013). Cell Metabolism. 18:660-671).
Western Blotting Analysis: A representative lot detected endogenous Mineralocorticoid Receptor (MR) in rat hippocampal cytosolic preparation and exogenously expressed EGFP-rat MR fusion protein in CHO cells (Gomez-Sanchez, C.E., et al. (2006). Endocrinology. 147(3):1343-1348).
Western Blotting Analysis: A representative lot detected endogenous Mineralocorticoid Receptor (MR) in mouse kidney cytosolic fraction and exogenously expressed human MR in COS-7 cells (Shibata, S., et al. (2013) Cell Metab. 18(5):660-671)
Biological Information
ImmunogenConjugated linear peptide corresponding to the N-terminus of rat Mineralocorticoid Receptor.
EpitopeNear N-terminus
Clone2D6
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityEpitope is present in all 3 alternative spliced isoforms
IsotypeIgG2aκ
Species Reactivity
  • Rat
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Nr3c2
  • Mlr
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~107 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in rat brain cytosol tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Mineralocorticoid Receptor in 10 µg of rat brain cytosol tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size200 μg
Transport Information
Supplemental Information
Specifications
Global Trade ITEM Number
Catalogue Number GTIN
MABS1250 04055977173031

Documentation

Anti-Mineralocorticoid Receptor Antibody, clone 2D6 SDS

Title

Safety Data Sheet (SDS) 

Anti-Mineralocorticoid Receptor Antibody, clone 2D6 Certificates of Analysis

TitleLot Number
Anti-Mineralocorticoid Receptor, -Q2573211 Q2573211
Anti-Mineralocorticoid Receptor, clone 2D6 - 3245604 3245604
Anti-Mineralocorticoid Receptor, clone 2D6 - 3884760 3884760
Anti-Mineralocorticoid Receptor, clone 2D6 - 4209927 4209927

Technical Info

Title
Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
White Paper: Further considerations of antibody validation and usage.