Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

DR1094 Anti-PAX7 Mouse mAb (1E12)

This Anti-PAX7 Mouse mAb (1E12) is validated for use in ELISA, Immunoblotting, Paraffin Sections for the detection of PAX7.

Anti-PAX7 Mouse mAb (1E12) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Paired Box 7

View Products on Sigmaaldrich.com
DR1094
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, MMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
DR1094-100UGCN 100 μg
Description
OverviewRecognizes the ~60 kDa PAX7 protein in NIH3T3 cells and human stomach tissue.
Catalogue NumberDR1094
Brand Family Calbiochem®
SynonymsAnti-Paired Box 7
Application Data
Detection of mouse PAX7 by immunoblotting. Sample: Whole cell lysate from NIH3T3 cells. Primary antibody: Anti-PAX7 Mouse mAb (1E12) (Cat. No. DR1094) (1-5 µg/ml). Detection: chemiluminescence.

Detection of mouse PAX7 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human stomach tissue. Primary antibody: Anti-PAX7 Mouse mAb (1E12) (Cat. No. DR1094) (0.5 µg/ml). Detection: colorimetric (DAB).
References
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
Positive controlNIH3T3 cells, Human stomach tissue
PreservativeNone
Quality SegmentMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Paraffin Sections
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Paraffin Sections (0.5 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenA recombinant polypeptide corresponding to amino acids of 411-521 human PAX7, expressed as a GST fusion protein
Clone1E12
HostMouse
IsotypeIgG2a
Species Reactivity
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade ITEM Number
Catalogue Number GTIN
DR1094-100UGCN 04055977226133

Documentation

Anti-PAX7 Mouse mAb (1E12) SDS

Title

Safety Data Sheet (SDS) 

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision04-November-2010 RFH
SynonymsAnti-Paired Box 7
ApplicationELISA (see comments)
Immunoblotting (1-5 µg/ml)
Paraffin Sections (0.5 µg/ml)
Application Data
Detection of mouse PAX7 by immunoblotting. Sample: Whole cell lysate from NIH3T3 cells. Primary antibody: Anti-PAX7 Mouse mAb (1E12) (Cat. No. DR1094) (1-5 µg/ml). Detection: chemiluminescence.

Detection of mouse PAX7 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human stomach tissue. Primary antibody: Anti-PAX7 Mouse mAb (1E12) (Cat. No. DR1094) (0.5 µg/ml). Detection: colorimetric (DAB).
DescriptionPurified mouse monoclonal antibody. Recognizes the ~60 kDa PAX7 protein.
BackgroundThis protein is a member of the paired box (PAX) family of transcription factors. Members of this family typically contain a paired box domain, an octapeptide, and a paired-type homeodomain. These proteins play critical roles during fetal development and cancer growth. The specific function of the paired box 7 gene is unknown but speculated to involve tumor suppression since fusion of this gene with a forkhead domain family member has been associated with alveolar rhabdomyosarcoma. Three transcript variants encoding different isoforms have been found for this gene.
HostMouse
ImmunogenA recombinant polypeptide corresponding to amino acids of 411-521 human PAX7, expressed as a GST fusion protein
Clone1E12
IsotypeIgG2a
Specieshuman, mouse
Positive controlNIH3T3 cells, Human stomach tissue
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review