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AB5210-50UL Anti-Sodium Channel Antibody, Voltage Gated, Pan (SP19 Segment)

This Sodium Channel antibody is validated for use in IHC & WB for the detection of the Sodium Channel protein.

Anti-Sodium Channel Antibody, Voltage Gated, Pan (SP19 Segment) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Ion Channels & Transporters, Neuronal & Glial Markers Gene Symbol: SCN1A, SCN1, HBSCI, Nav1.1, FEB3, NAC1, SMEI, GEFSP2 UniProt Number: P35498
AB5210-50UL
50 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, RIHC, WBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB5210-50UL
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Sodium Channel Antibody, Voltage Gated, Pan (SP19 Segment)
References
Product Information
FormatAffinity Purified
Control
  • CONTROL ANTIGEN: Included free of charge with the antibody is 40 μg of control antigen. The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
PresentationAffinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA and 0.05% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
Quality SegmentMQ100
Applications
ApplicationThis Sodium Channel antibody is validated for use in IHC & WB for the detection of the Sodium Channel protein.
Key Applications
  • Immunohistochemistry
  • Western Blotting
Application NotesAll procedures that are going to receive a full-length protein should be performed at 4C, and the following protease inhibitor mixture should be used: pepstatin A (1 μg/mL), leupeptin (1 μg/mL), aprotinin (1 μg/mL), Pefabloc SC (0.2 mM), benzamidine (0.1 mg/mL), and calpain inhibitors I and II (8 μg/mL each).

Western blot: 1:200 using ECL on newborn rat brain membranes.

Immunohistochemistry on mouse brain sections (frozen) and mouse spinal cord sections (paraffin).

Dilutions should be made using a carrier protein such as BSA (1-3%)

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenPurified peptide (SP19) of alpha subunit (amino acids 1501-1518) of rat type I voltage-gated sodium channel (VGSC) (Accession P04774).
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityRecognizes alpha subunits of VGSC (Pan Nav SP19). Does not cross react with any other sodium channel antigens tested so far.a
Species Reactivity
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe vertebrate sodium channel is a voltage-gated ion channel essential for the generation and propagation of action potentials, chiefly in nerve and muscle. Voltage-sensitive sodium channels are heteromeric complexes consisting of a large central pore-forming glycosylated alpha subunit and 2 smaller auxiliary beta subunits. Functional studies have indicated that the transmembrane alpha subunit of the brain sodium channels is sufficient for expression of functional sodium channels (Goldin et al., 1986 [PubMed 2429308]; Isom, 2002 [PubMed 11779698]).[supplied by OMIM]
Gene Symbol
  • SCN1A
  • SCN1
  • HBSCI
  • Nav1.1
  • FEB3
  • NAC1
  • SMEI
  • GEFSP2
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P35498 # Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient.
SIZE: 2009 amino acids; 228972 Da
SUBUNIT: The sodium channel consists of a large polypeptide and 2- 3 smaller ones. This sequence represents a large polypeptide.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
DOMAIN: SwissProt: P35498 The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position.
DISEASE: SwissProt: P35498 # Defects in SCN1A are the cause of generalized epilepsy with febrile seizures plus type 2 (GEFS+2) [MIM:604233]. This autosomal dominant disorder is characterized by febrile seizures in children and afebrile seizures in adults. Penetrance is incomplete and a large intrafamilial variability of the phenotype is observed. & Defects in SCN1A are a cause of severe myoclonic epilepsy in infancy (SMEI) [MIM:607208]; also called Dravet syndrome. SMEI is a severe form of generalized epilepsy with febrile seizures. It is a rare disorder characterized by normal development before onset, seizures beginning in the first year of life in the form of generalized or unilateral febrile clonic seizures, secondary appearance of myoclonic seizures, and occasionally partial seizures. It is associated with ataxia, slowed psychomotor development, and mental decline. & Defects in SCN1A are the cause of familial hemiplegic migraine 3 (FHM3) [MIM:609634]. FHM3 is an autosomal dominant severe subtype of migraine with aura characterized by some degree of hemiparesis during the attacks. The episodes are associated with variable features of nausea, vomiting, photophobia and phonophobia. Age at onset ranges from 6 to 15 years. Some patients may manifest seizures during infancy. & Defects in SCN1A are the cause of familial febrile convulsions 3 (FEB3) [MIM:604403]; also known as familial febrile seizures 3. Febrile convulsions affect 5-12% of infants and children up to 6 years of age. There is epidemiological evidence that febrile seizures are associated with subsequent afebrile and unprovoked seizures in 2% to 7% of patients. Inheritance pattern is autosomal dominant.
SIMILARITY: Belongs to the sodium channel family. & Contains 1 IQ domain.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size50 µL
Transport Information
Supplemental Information
Specifications
Global Trade ITEM Number
Catalogue Number GTIN
AB5210-50UL 04053252742187

Documentation

Anti-Sodium Channel Antibody, Voltage Gated, Pan (SP19 Segment) SDS

Title

Safety Data Sheet (SDS) 

Anti-Sodium Channel Antibody, Voltage Gated, Pan (SP19 Segment) Certificates of Analysis

TitleLot Number
RABBIT ANTI-PAN VOLTAGE GATED SODIUM CHANNEL, SP19 AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2379291 2379291
RABBIT ANTI-PAN VOLTAGE GATED SODIUM CHANNEL, SP19 AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2159620 2159620
RABBIT ANTI-PAN VOLTAGE GATED SODIUM CHANNEL, SP19 - 3244064 3244064
RABBIT ANTI-PAN VOLTAGE GATED SODIUM CHANNEL, SP19 - 3417911 3417911
RABBIT ANTI-PAN VOLTAGE GATED SODIUM CHANNEL, SP19 - 3494096 3494096
RABBIT ANTI-PAN VOLTAGE GATED SODIUM CHANNEL, SP19 -2698391 2698391
RABBIT ANTI-PAN VOLTAGE GATED SODIUM CHANNEL, SP19 -2799100 2799100
RABBIT ANTI-PAN VOLTAGE GATED SODIUM CHANNEL, SP19 AFFINITY PURIFIED POLYCLONAL ANTIBODY 3105784
RABBIT ANTI-PAN VOLTAGE GATED SODIUM CHANNEL, SP19 AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2207136 2207136
RABBIT ANTI-PAN VOLTAGE GATED SODIUM CHANNEL, SP19 AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2274245 2274245

Data Sheet

Title
RABBIT ANTI-PAN VOLTAGE GATED SODIUM CHANNEL, SP19 AFFINITY PURIFIED POLYCLONAL ANTIBODY

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Alternative Packsize

Catalogue Number Description
AB5210 Anti-Sodium Channel Antibody, Voltage Gated, Pan (SP19 Segment)

Categories

Life Science Research > Antibodies and Assays > Primary Antibodies