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05-1461 Anti-Src Family (WTAPE) Antibody, clone 2E8.2

Use Anti-Src Family (WTAPE) Antibody, clone 2E8.2 (Mouse Monoclonal Antibody) validated in WB to detect Src Family (WTAPE) also known as Integrin-linked kinase, 59 kDa serine/threonine-protein kinase.

Anti-Src Family (WTAPE) Antibody, clone 2E8.2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Cytoskeletal Signaling, Kinases & Phosphatases Gene Symbol: pp60c-src, p60-Src, c-Src UniProt Number: P12931
05-1461
200 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RWBMPurifiedMonoclonal Antibody
Description
Catalogue Number05-1461
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-Src Family (WTAPE) Antibody, clone 2E8.2
Alternate Names
  • Integrin-linked kinase
  • 59 kDa serine/threonine-protein kinase
Background Informationpp60c-Src (Src) is the prototype non-receptor tyrosine kinase Src was the first proto-oncogenic protein discovered. The 9 members of the Src family include Src, Lyn, Fyn, Yes, Lck, Hck, Blk, Fgr, and Yrk. They share common structural characteristics that include an N-terminal anchor sequence that allows the protein to the membrane followed by both an SH3 and an SH2 domain, a catalytic domain, an activation loop, and finally a C-term region that binds to the SH2 domain when phosphorylated (Tyr527 in Src that is phosphorylated by Csk). This binding causes Src to adopt an inactive conformation. Mutation of Tyr527 to phenylalanine removes the SH2-binding target, and results in a constitutively active kinase. A second mutation at the conserved lysine residue at the ATP-binding site abolishes kinase activity, but without Tyr527, the conformation of the protein is open, allowing interaction with endogenous activators. Consequently, this double-mutant exhibits a dominant-negative phenotype. Homology to Src in three regions is shared by a number of proteins, SH1 being the catalytic domain, SH2 being a phospho-tyrosine binding domain, and SH3 being a proline-binding domain.
References
Product Information
FormatPurified
Control
  • A431 Cell Lysate
PresentationProtein G purified mouse monoclonal in PBS and 0.05% NaN3.
Quality SegmentMQ100
Applications
ApplicationUse Anti-Src Family (WTAPE) Antibody, clone 2E8.2 (Mouse Monoclonal Antibody) validated in WB to detect Src Family (WTAPE) also known as Integrin-linked kinase, 59 kDa serine/threonine-protein kinase.
Key Applications
  • Western Blotting
Biological Information
ImmunogenShared Src family sequence found at the C-term. Amino acid sequence GAKFPIKWTAPE.
EpitopeC-term WTAPE region
Clone2E8.2
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityRecognizes Src Family Members at the following amino acids:
Src:424-435, Lyn:402-413, Fyn:425-436, Yes:431-442, Lck:399-411, Hck:394-405, Blk:394-405, and Fgr: 417-428.
IsotypeIgG3κ
Species Reactivity
  • Human
  • Mouse
  • Rat
Species Reactivity NoteHuman and Rat tested. Expected to react with Mouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummarySummary for proto-oncogene tyrosine-protein kinase SRC [Homo sapiens] (accession no. NP_005408) (one example of Src family proteins containing the immunogen sequence: other members include Src, Lyn, Fyn, Yes, Lck, Hck, Blk, Fgr, and Yrk).
The gene for the proto-oncogene tyrosine-protein kinase SRC [Homo sapiens] is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq].
Gene Symbol
  • pp60c-src
  • p60-Src
  • c-Src
Purification MethodProtein G Purified
UniProt Number
UniProt SummarySummary for SRC_HUMAN (accession no. P12931) (one example of Src family proteins containing the immunogen sequence: other members include Lyn, Fyn, Yes, Lck, Hck, Blk, Fgr, and Yrk).
CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
SUBUNIT: Interacts with DDEF1/ASAP1; via the SH3 domain. Interacts with CCPG1 (By similarity). Interacts with CDCP1, PELP1, TGFB1I1 and TOM1L2. Interacts with the cytoplasmic domain of MUC1, phosphorylates it and increases binding of MUC1 with beta-catenin. Interacts with RALGPS1; via the SH3 domain. Interacts with HEV ORF3 protein; via the SH3 domain.
PTM: Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. The phosphorylated tail interacts with the SH2 domain thereby repressing kinase activity.
SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
SIMILARITY: Contains 1 protein kinase domain.
SIMILARITY: Contains 1 SH2 domain.
SIMILARITY: Contains 1 SH3 domain.
Molecular Weight~60 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by Western Blot
Western Blot Analysis:
This lot detected Src family members at a 2μg/mL dilution in EGF-treated A431 cell lysate resolved via SDS-PAGE and transferred to PVDF (Immobilon-P).
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance
Packaging Information
Material Size200 µg
Transport Information
Supplemental Information
Specifications
Global Trade ITEM Number
Catalogue Number GTIN
05-1461 04053252323782

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Categories

Life Science Research > Antibodies and Assays > Primary Antibodies