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AB5600-50UL Anti-Synaptotagmin I Antibody, luminal part

Detect Synaptotagmin I using this Anti-Synaptotagmin I Antibody, luminal part validated for use in WB, IH.

Anti-Synaptotagmin I Antibody, luminal part MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Synapse & Synaptic Biology Gene Symbol: SYT1, DKFZp781D2042, SVP65, SytI, SYT, p65, P65, Synaptotagmin-1 UniProt Number: P21579
AB5600-50UL
50 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
RWB, IHCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB5600-50UL
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Synaptotagmin I Antibody, luminal part
Alternate Names
  • p65
  • Syt1
References
Product Information
FormatAffinity Purified
Control
  • CONTROL ANTIGEN: Included free of charge with the antibody is 20 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
PresentationAffinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 5% sucrose as a stabilizer and 0.025% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
Quality SegmentMQ100
Applications
ApplicationDetect Synaptotagmin I using this Anti-Synaptotagmin I Antibody, luminal part validated for use in WB, IH.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application NotesWestern blot: 1:200-1:1,000 using ECL on rat brain membranes.

Immunohistochemistry on rat brain sections.

Dilutions should be made using a carrier protein such as BSA (1-3%)

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenPeptide corresponding to amino acids 1-19 from rat Synaptotagmin I (Accession # P21107).
Epitopeluminal part
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityRecognizes Synaptotagmin I, luminal part. The epitope does not share homology with any other known proteins.

SPECIES REACTIVITIES: It is expected that the antibody will also recognize human. Other species have not been tested.
Species Reactivity
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin I participates in triggering neurotransmitter release at the synapse (Fernandez-Chacon et al., 2001 [PubMed 11242035]).[supplied by OMIM]
Gene Symbol
  • SYT1
  • DKFZp781D2042
  • SVP65
  • SytI
  • SYT
  • p65
  • P65
  • Synaptotagmin-1
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P21579 # May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2.
COFACTOR: Binds 3 calcium ions per subunit. The ions are bound to the C2 domains (By similarity).
SIZE: 422 amino acids; 47573 Da
SUBUNIT: Homotetramer (Probable). Interacts with STN2, SV2A, SV2B, SV2C and RIMS1. Forms a complex with SV2B, syntaxin 1 and SNAP25 (By similarity).
SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle. Cytoplasmic vesicle, secretory vesicle, chromaffin granule. Note=Synaptic vesicles and chromaffin granules.
DOMAIN: SwissProt: P21579 The first C2 domain mediates Ca(2+)-dependent phospholipid binding. & The second C2 domain mediates interaction with SV2A and STN2 (By similarity).
SIMILARITY: Belongs to the synaptotagmin family. & Contains 2 C2 domains.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain lyophilized material at -20°C for up to 12 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size50 µL
Transport Information
Supplemental Information
Specifications
Global Trade ITEM Number
Catalogue Number GTIN
AB5600-50UL 04053252407666

Documentation

Anti-Synaptotagmin I Antibody, luminal part SDS

Title

Safety Data Sheet (SDS) 

Anti-Synaptotagmin I Antibody, luminal part Certificates of Analysis

TitleLot Number
RABBIT ANTI-SYNAPTOTAGMIN I AFFINITY PURIFIED, POLYCLONAL ANTIBODY - 2139958 2139958
RABBIT ANTI-SYNAPTOTAGMIN I AFFINITY PURIFIED, POLYCLONAL ANTIBODY - 2141800 2141800
RABBIT ANTI-SYNAPTOTAGMIN I AFFINITY PURIFIED, POLYCLONAL ANTIBODY - 2370093 2370093
RABBIT ANTI-SYNAPTOTAGMIN I - 2492211 2492211
RABBIT ANTI-SYNAPTOTAGMIN I - 3398588 3398588
RABBIT ANTI-SYNAPTOTAGMIN I -2608840 2608840
RABBIT ANTI-SYNAPTOTAGMIN I -2638066 2638066
RABBIT ANTI-SYNAPTOTAGMIN I -2689077 2689077
RABBIT ANTI-SYNAPTOTAGMIN I -2705108 2705108
RABBIT ANTI-SYNAPTOTAGMIN I -2706067 2706067
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Brochure

Title
Pathways and Biomarkers of Glutamatergic Synapse Flyer

Data Sheet

Title
RABBIT ANTI-SYNAPTOTAGMIN I AFFINITY PURIFIED, POLYCLONAL ANTIBODY