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ABE1029 Anti-acetyl Histone H3 Antibody (Lys122)

Anti-acetyl Histone H3 (Lys122) is an antibody against acetyl Histone H3 (Lys122) for use in WB, ICC, DB, IP.

Anti-acetyl Histone H3 Antibody (Lys122) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: HIST1H3A, H3FA, HIST1H3B, H3FL, HIST1H3C, H3FC, HIST1H3D, H3FB, HIST1H3E, H3FD, HIST1H3F, H3FI, HIST1H3G, H3FH, HIST1H3H, H3FK, HIST1H3I, H3FF, HIST1H3J, H3FJ UniProt Number: P68431
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ABE1029
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, ICC, DB, IPRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABE1029
DescriptionAnti-acetyl Histone H3 Antibody (Lys122)
Alternate Names
  • Histone H3.1
  • Histone H3/a
  • Histone H3/b
  • Histone H3/c
  • Histone H3/d
  • Histone H3/f
  • Histone H3/h
  • Histone H3/I
  • Histone H3/j
  • Histone H3/k
  • Histone H3/l
Background InformationHistone H3, also known as Histone H3.1t (H3/t), H3t, H3/g, and encoded by the gene name HIST3H3/ H3FT, is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Therefore, histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. High levels of phosphorylation of Histone H3 are associated with mitosis.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality SegmentMQ100
Applications
ApplicationAnti-acetyl Histone H3 (Lys122) is an antibody against acetyl Histone H3 (Lys122) for use in WB, ICC, DB, IP.
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Dot Blot
  • Immunoprecipitation
Application NotesImmunocytochemistry Analysis: A 1:250 dilution from a representative lot detected acetyl Histone H3 (Lys122) in HeLa and A431cells.
Dot Blot Analysis: A 1:1,000 dilution from a representative lot detected acetyl Histone H3 (Lys122) in Histone peptides with various modifications.
Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated acetyl Histone H3 (Lys122) in 500 µg of sodium butyrate treated HeLa acid extract.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human acetyl Histone H3 (Lys122) near the C-terminal.
EpitopeNear C-terminal
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
Species Reactivity
  • Human
  • Mouse
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • HIST1H3A
  • H3FA
  • HIST1H3B
  • H3FL
  • HIST1H3C
  • H3FC
  • HIST1H3D
  • H3FB
  • HIST1H3E
  • H3FD
  • HIST1H3F
  • H3FI
  • HIST1H3G
  • H3FH
  • HIST1H3H
  • H3FK
  • HIST1H3I
  • H3FF
  • HIST1H3J
  • H3FJ
Modifications
  • Acetylation
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~17 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in sodium butyrate treated HeLa acid extract.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected acetyl Histone H3 (Lys122) in 10 µg of sodium butyrate treated HeLa acid extract.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade ITEM Number
Catalogue Number GTIN
ABE1029 04055977115826