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07-829 Anti-phospho-FAK (Tyr407) Antibody

Anti-phospho-FAK (Tyr407) Antibody is an antibody against phospho-FAK (Tyr407) for use in WB.

Anti-phospho-FAK (Tyr407) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Cytoskeletal Signaling Gene Symbol: PTK2, pp125FAK, FAK1, FADK, FAK UniProt Number: Q05397
07-829
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number07-829
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-phospho-FAK (Tyr407) Antibody
Alternate Names
  • PTK2
  • FADK
  • FAK
  • FAK1
  • pp125FAK
  • Focal Adhesion Kinase 1
References
Product Information
FormatAffinity Purified
Applications
ApplicationAnti-phospho-FAK (Tyr407) Antibody is an antibody against phospho-FAK (Tyr407) for use in WB.
Key Applications
  • Western Blotting
Application NotesOutside independent labs report it working in immunocytochemistry and immunohistochemistry.
Biological Information
ImmunogenPeptide corresponding to amino acid region encompassing the human, mouse, rat, and chicken phospho-FAK (Tyr407)
HostRabbit
SpecificityRecognizes phosphor-FAK (Tyr407)
IsotypeIgG
Species Reactivity
  • Human
Species Reactivity NotePredicted to cross-react with Mouse, Rat, and Chicken based on complete sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. At least four transcript variants encoding four different isoforms have been found for this gene, but the full-length natures of only two of them have been determined.
Gene Symbol
  • PTK2
  • pp125FAK
  • FAK1
  • FADK
  • FAK
Modifications
  • Phosphorylation
Purification MethodImmunoAffinity Purified
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q05397 # Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
SIZE: 1052 amino acids; 119233 Da
SUBUNIT: Interacts with CAS family members and with GIT1, SORBS1 and BCAR3. Interacts with SHB (By similarity). Interacts with TGFB1I1.
SUBCELLULAR LOCATION: Cell junction, focal adhesion; Cytoplasmic side. Note=Constituent of focal adhesions.
TISSUE SPECIFICITY: Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.
DOMAIN: SwissProt: Q05397 The first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL (By similarity). & The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions.
PTM: Phosphorylated on 6 tyrosine residues upon activation.
SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily. & Contains 1 FERM domain. & Contains 1 protein kinase domain.
Molecular Weight125 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by immunoblot.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions2 years at -20°C
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade ITEM Number
Catalogue Number GTIN
07-829 04053252676536

Documentation

Anti-phospho-FAK (Tyr407) Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-phospho-FAK (Tyr407) Antibody Certificates of Analysis

TitleLot Number
Anti-phospho-FAK (Tyr407) (Affinity purified Rabbit polyclonal IgG) - NG1944007 NG1944007
anti-phospho-FAK (Tyr407) - 0605030964 0605030964

References

Reference overviewPub Med ID
Regulation of vascular endothelial growth factor receptor 2-mediated phosphorylation of focal adhesion kinase by heat shock protein 90 and Src kinase activities
Le Boeuf, Fabrice, et al
J Biol Chem, 279:39175-85 (2004)  2004

15247219 15247219
Expression of GRP and its receptor in well-differentiated colon cancer cells correlates with the presence of focal adhesion kinase phosphorylated at tyrosines 397 and 407
Matkowskyj, Kristina A, et al
J Histochem Cytochem, 51:1041-8 (2003)  2003

12871985 12871985

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Categories

Life Science Research > Antibodies and Assays > Primary Antibodies