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07-815 Anti-phospho-Raf-1 (Tyr340/Tyr341) Antibody

Detect phospho-Raf-1 (Tyr340/Tyr341) using this Anti-phospho-Raf-1 (Tyr340/Tyr341) Antibody validated for use in WB.

Anti-phospho-Raf-1 (Tyr340/Tyr341) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: MAP Kinases Gene Symbol: RAF1, C-RAF, cRaf, Raf-1, c-Raf, CRAF, RAF UniProt Number: P04049
07-815
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number07-815
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-phospho-Raf-1 (Tyr340/Tyr341) Antibody
Alternate Names
  • CRAF
  • Raf1
  • c-Raf
References
Product Information
FormatAffinity Purified
Applications
ApplicationDetect phospho-Raf-1 (Tyr340/Tyr341) using this Anti-phospho-Raf-1 (Tyr340/Tyr341) Antibody validated for use in WB.
Key Applications
  • Western Blotting
Application NotesPredicted to cross-react with Mouse and Rat based on complete sequence homology.
Biological Information
ImmunogenPeptide corresponding to amino acid region encompassing the human, mouse, and rat phospho-Raf-1 (Tyr340/341).
HostRabbit
SpecificityRecognizes phospho-Raf-1 (Tyr340/341)
IsotypeIgG
Species Reactivity
  • Human
Species Reactivity NotePredicted to cross-react with Mouse and Rat based on complete sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryRaf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. [Contributed text]
Gene Symbol
  • RAF1
  • C-RAF
  • cRaf
  • Raf-1
  • c-Raf
  • CRAF
  • RAF
Modifications
  • Phosphorylation
Purification MethodImmunoAffinity Purified
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P04049 # Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.
COFACTOR: Binds 2 zinc ions per subunit.
SIZE: 648 amino acids; 73052 Da
SUBUNIT: Interacts with Ras proteins. The interaction with Ras is antagonized by RIN1. Weakly interacts with RIT1 (By similarity). Interacts with STK3, which inhibits its pro-apoptotic activity.
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.SIMILARITY:SwissProt: P04049 ## Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily. & Contains 1 phorbol-ester/DAG-type zinc finger. & Contains 1 protein kinase domain. & Contains 1 RBD (Ras-binding) domain.
Molecular Weight74 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by immunoblot.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions2 years at -20°C
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade ITEM Number
Catalogue Number GTIN
07-815 04053252369018

Documentation

Anti-phospho-Raf-1 (Tyr340/Tyr341) Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-phospho-Raf-1 (Tyr340/Tyr341) Antibody Certificates of Analysis

TitleLot Number
Anti-phospho-Raf-1 (Tyr340/341) - 2013514 2013514
Anti-phospho-Raf-1 (Tyr340/341) (Affinity purified Rabbit polyclonal IgG) - 2386934 2386934
Anti-phospho-Raf-1 (Tyr340/341) (Affinity purified Rabbit polyclonal IgG) - 2159616 2159616
Anti-phospho-Raf-1 (Tyr340/341) (Affinity purified Rabbit polyclonal IgG) - DAM1798675 DAM1798675
Anti-phospho-Raf-1 (Tyr340/341) (Affinity purified Rabbit polyclonal IgG) - NG1926736 NG1926736
Anti-phospho-Raf-1 (Tyr340/341) - 1982452 1982452
Anti-phospho-Raf-1 (Tyr340/341) - DAM1411305 DAM1411305
Anti-phospho-Raf-1 (Tyr340/341) - DAM1498501 DAM1498501
Anti-phospho-Raf-1 (Tyr340/341) - DAM1717402 DAM1717402
Anti-phospho-Raf-1 (Tyr340/341) - JBC1863390 JBC1863390
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References

Reference overviewPub Med ID
Dynamic changes in C-Raf phosphorylation and 14-3-3 protein binding in response to growth factor stimulation: differential roles of 14-3-3 protein binding sites
Hekman, Mirko, et al
J Biol Chem, 279:14074-86 (2004)  2004

14688280 14688280
Positive and negative regulation of Raf kinase activity and function by phosphorylation
Chong, H, et al
EMBO J, 20:3716-27 (2001)  2001

11447113 11447113