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14-458M CSK Protein, active, 250 µg

Active, recombinant full-length human CSK, containing an N-terminal GST-tag incorporating a PreScission Protease cleavage site, for use in Kinase Assays.

CSK Protein, active, 250 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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14-458M
250 µg  Also available in 10 μg size (cat#14-458) and in bulk (cat# 14-458-K).
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Overview

Replacement Information
Description
Catalogue Number14-458M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionCSK Protein, active, 250 µg
OverviewRecombinant full-length human CSK, containing an N-terminal GST-tag incorporating a PreScission Protease cleavage site
References
Product Information
Quality SegmentMQ100
Applications
ApplicationActive, recombinant full-length human CSK, containing an N-terminal GST-tag incorporating a PreScission Protease cleavage site, for use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
SourceExpressed in E. coli BL21 (DE3) cells
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Gene Symbol
  • CSK
  • MGC117393
Protein TargetCSK
Purification MethodGSH-agarose chromatography
Target Sub-FamilyTK
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P41240 # Specifically phosphorylates 'Tyr-504' on LCK, which acts as a negative regulatory site. Can also act on the LYN and FYN kinases.
SIZE: 450 amino acids; 50704 Da
SUBUNIT: Interacts with PTPN8 (By similarity). Interacts with phosphorylated SIT1, PAG1, LIME1 and TGFB1I1.
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Cell membrane (By similarity). Note=Mainly cytoplasmic, also present in lipid rafts (By similarity).
TISSUE SPECIFICITY: Expressed in lung and macrophages.
PTM: Autophosphorylation of Tyr-304 occurs only at abnormally high CSK concentrations in vitro.
SIMILARITY: SwissProt: P41240 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily. & Contains 1 protein kinase domain. & Contains 1 SH2 domain. & Contains 1 SH3 domain.
Molecular Weight77.6kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by phosphorylation of Poly (Glu4Tyr) substrate peptide
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions1 year at -20°C
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-458) and in bulk (cat# 14-458-K).
Transport Information
Supplemental Information
Specifications
Global Trade ITEM Number
Catalogue Number GTIN
14-458M 04053252336744

Related Products & Applications

Alternative Packsize

Catalogue Number Description
14-458 CSK Protein, active, 10 µg

Product Families

Kinase & Phosphatase Products

Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
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Categories

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases