Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

14-519M Cdk6/Cyclin D3 Protein, active, 250 µg

Active, N-terminal, His6-tagged full length human Cdk6 complexed with N-terminal GST-tagged full-length human Cyclin D3, for use in Kinase Assays.

Cdk6/Cyclin D3 Protein, active, 250 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
View Products on Sigmaaldrich.com
14-519M
250 µg  Also available in 10 μg size (cat#14-519) and in bulk (cat# 14-519-K).
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number14-519M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionCdk6/Cyclin D3 Protein, active, 250 µg
OverviewN-terminal, His6-tagged fulllength human cdk6 complexed with N-terminal GSTtagged full-length human cyclin D3
References
Product Information
Quality SegmentMQ100
Applications
ApplicationActive, N-terminal, His6-tagged full length human Cdk6 complexed with N-terminal GST-tagged full-length human Cyclin D3, for use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
Sourceexpressed in Sf21 cells
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb.
Gene Symbol
  • CDK6
  • PLSTIRE
  • MGC59692
Protein TargetCDK6/cyclinD3
Purification MethodGlutathione-agarose
Target Sub-FamilyCMGC
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q00534 # Probably involved in the control of the cell cycle. Interacts with D-type G1 cyclins.
SIZE: 326 amino acids; 36938 Da
SIMILARITY: SwissProt: Q00534 ## Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. & Contains 1 protein kinase domain.
Molecular Weight38kDa (cdk6) and 59kDa (cyclin D3)
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by phosphorylation of histone H1
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70°C
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-519) and in bulk (cat# 14-519-K).
Transport Information
Supplemental Information
Specifications
Global Trade ITEM Number
Catalogue Number GTIN
14-519M 04053252333774

Related Products & Applications

Alternative Packsize

Catalogue Number Description
14-519 Cdk6/Cyclin D3 Protein, active, 10 µg

Product Families

Kinase & Phosphatase Products

Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
Learn More >>

Categories

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases