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14-659 LIM Kinase 1 Protein, inactive, 50 µg

Inactive, N-terminal 6His-tagged, recombinant, human LIM Kinase 1, amino acids 285-638. For use in Kinase Assays.

LIM Kinase 1 Protein, inactive, 50 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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14-659
50 µg  
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Overview

Replacement Information
Description
Catalogue Number14-659
Brand Family Upstate
Trade Name
  • Upstate
DescriptionLIM Kinase 1 Protein, inactive, 50 µg
OverviewN-terminal 6His-tagged, recombinant, human LIM Kinase 1, amino acids 285-638.
References
Product Information
Quality SegmentMQ100
Applications
ApplicationInactive, N-terminal 6His-tagged, recombinant, human LIM Kinase 1, amino acids 285-638. For use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
SourceExpressed by baculovirus in Sf21 cells.
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryThere are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is likely to be a component of an intracellular signaling pathway and may be involved in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome.
Gene Symbol
  • LIMK1
  • LIMK
  • LIMK-1
Protein TargetLIMK1
Purification MethodNi2+/NTA-agarose
Target Sub-FamilyTKL
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P53667 # Protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. May be involved in brain development.
SIZE: 647 amino acids; 72585 Da
SUBUNIT: Self-associates. The LIM domain interacts with the cytoplasmic domain of NRG1. Binds ROCK1. Interacts with SSH1.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Highest expression in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle.
PTM: Autophosphorylated (By similarity). & Phosphorylated on serine and/or threonine residues by ROCK1. May be dephosphorylated and inactivated by SSH1.
DISEASE: SwissProt: P53667 # Haploinsufficiency of LIMK1 may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in Williams-Beuren syndrome (WBS), a rare developmental disorder. It is a contiguous gene deletion syndrome involving genes from chromosome band 7q11.23.
SIMILARITY: SwissProt: P53667 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. & Contains 2 LIM zinc-binding domains. & Contains 1 PDZ (DHR) domain. & Contains 1 protein kinase domain.
Molecular Weight41.8kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by phosphorylation of Cofilin 1 (Catalog # 12-556).
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70°C from date of shipment
Packaging Information
Material Size50 µg
Transport Information
Supplemental Information
Specifications
Global Trade ITEM Number
Catalogue Number GTIN
14-659 04053252734502