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33-039 PI 3-Kinase Class II HTRF Assay

Five-plate kit (1920 wells) of reagents for performing PI 3-Kinase (Class II) 4-step HTRF assays. Suitable for the following Kinases: PI3KC2α, PI3KC2β & PI3KC2γ.

PI 3-Kinase Class II HTRF Assay MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
33-039
1920 wells  Kit capacity: 1920 wells
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number33-039
Brand Family Upstate
Trade Name
  • HTRF
  • Upstate
DescriptionPI 3-Kinase Class II HTRF Assay
References
Product Information
Components
  • Reaction Buffer (4X)
  • DM A Detection Reagent
  • DM C Detection Reagent
  • DM D Detection Reagent
  • Phosphatidylinositol (1mM)
  • Phosphatidylserine (1mM)
  • Stop Reagent C
Detection methodHTRF
Quality SegmentMQ300
Applications
ApplicationFive-plate kit (1920 wells) of reagents for performing PI 3-Kinase (Class II) 4-step HTRF assays. Suitable for the following Kinases: PI3KC2α, PI3KC2β & PI3KC2γ.
Key Applications
  • Kinase Assay
Biological Information
Species Reactivity
  • Human
  • Mouse
Analytes Available
  • PI 3-Kinase Class II
Entrez Gene Number
Entrez Gene SummaryPhosphoinositide 3-kinase-C2 proteins generate phosphatidylinositol 3-phosphate (PtdIns3P) and phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4)P2) that act as second messengers. This protein belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. The PI3-kinase activity of this protein is sensitive to low nanomolar levels of the inhibitor wortmanin. The C2 domain of this protein was shown to bind phospholipids but not Ca2+, which suggests that this enzyme may function in a calcium-independent manner.
Gene Symbol
  • PI3KC2α
  • PI3KC2β
  • PI3KC2γ
Target Sub-FamilyPI3K/PI4K
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions12 months at -70°C
Packaging Information
Material Size1920 wells
Material PackageKit capacity: 1920 wells
Transport Information
Supplemental Information
Specifications
Global Trade ITEM Number
Catalogue Number GTIN
33-039 04053252374548

Related Products & Applications

Alternative Packsize

Catalogue Number Description
33-038 PI 3-Kinase Class II HTRF Assay

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Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Enzyme Assay Kits and Reagents