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MABT1240 Sigma-AldrichAnti-Cathepsin G Antibody, clone AHN-11, Ascites Free

This Anti-Cathepsin G Antibody, clone AHN-11, Ascites Free is validated for use in Immunocytochemistry, Western Blotting, ELISA, Radioimmunoassay for the detection of Cathepsin G.

Anti-Cathepsin G Antibody, clone AHN-11, Ascites Free MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
分析证书

Research Category: Cell Structure Research Sub-Category: Inflammation & Autoimmune Mechanisms Gene Symbol: CTSG UniProt Number: P08311

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概述

Replacement Information

特卖

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重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	ICC, WB, ELISA, RIA	M	Purified	Monoclonal Antibody

Description
Catalogue Number	MABT1240
Replaces	MAB1054
Description	Anti-Cathepsin G Antibody, clone AHN-11, Ascites Free
Alternate Names	Cathepsin G CatG CG
Background Information	Cathepsin G (EC 3.4.21.20; UniProt P08311; also known as CatG, CG) is encoded by the CTSG gene (Gene ID 1511) in human. Cathepsin G is a peptidase S1 protein family protease found in azurophil granules of neutrophilic polymorphonuclear leukocytes. Cathepsin G displays a substrate specificity similar to that of chymotrypsin C, but it is most closely related to other immune serine proteases, such as neutrophil elastase and the granzymes. Cathepsin G is involved in connective tissue remodeling at sites of inflammation. Cathepsin G is also a component of neutrophil extracellular traps (NETs) composed of ejected lattice of chromatin enmeshed with granular and nuclear proteins, including histones, calprotectin and cathepsin G, that are capable of capturing and killing microbial invaders. Antibodies raised against cathepsin G or histones have been shown to limit the bactericidal capacity of NETs towards pathogens. Cathepsin G is prouduced with a signal peptide (a.a. 1-18) and a propeptide sequence (a.a. 19-20) that are removed posttranslationally to yield the mature (a.a. 21-255) enzyme.

References

Product Information
Format	Purified
Presentation	Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Segment	MQ100

Applications
Application	This Anti-Cathepsin G Antibody, clone AHN-11, Ascites Free is validated for use in Immunocytochemistry, Western Blotting, ELISA, Radioimmunoassay for the detection of Cathepsin G.
Key Applications	Immunocytochemistry Western Blotting ELISA Radioimmunoassay
Application Notes	Western Blotting Analysis: A representative lot detected cathepsin G in the primary granule fraction from human neutrophils (Stroncek D.F., et al. (2005). J. Transl. Med. 3:36). Immunocytochemistry Analysis: Clone AHN-11 hybridoma culture supernatant immunostained ethanol-fixed human neutrophils, but not other peripheral blood cells, including platelets, lymphocytes, and eosinophils. Faint immunostaining of some monocytes was occasionally detected (Skubitz, K.M., et al. (1989). J. Leukoc. Biol. 46(2):109-118). ELISA Analysis: A representative lot captured cathepsin G from human neutrophil granule extract. The captured cathepsin G served to detect the presence of serum cathepsin G autoantibody levels in patients serum samples (Ellerbroek, P.M., et al. (1994). J. Clin. Pathol. 47(3):257-262). Radioimmunoassay Analysis: Clone AHN-11 hybridoma culture supernatant bound to well surface coated with dried human neutrophils, but not platelets or erythrocytes by solid-phase radioimmunoassay (Skubitz, K.M., et al. (1989). J. Leukoc. Biol. 46(2):109-118). Radioimmunoassay Analysis: Clone AHN-11 hybridoma culture supernatant bound to well surface coated with content of primary granule, but not secondary granule, plasma membrane, or cytoplasm fraction from neutrophils by solid-phase radioimmunoassay (Skubitz, K.M., et al. (1989). J. Leukoc. Biol. 46(2):109-118). Radioimmunoassay Analysis: Clone AHN-11 hybridoma culture supernatant reacted with purified human cathepsin G, but not elastase, EPO, esterase N, proteinase 3, plamin, kallidrein, lactoferrin, MBP, or thrombin by solid-phase radioimmunoassay (Skubitz, K.M., et al. (1989). J. Leukoc. Biol. 46(2):109-118).

Biological Information
Immunogen	Human neutrophils.
Clone	AHN-11
Concentration	Please refer to lot specific datasheet.
Host	Mouse
Specificity	Clone AHN-11 reacted specifically with neutrophil cathepsin G, but not the homologous neutrophil neutral proteases, elastase, proteinase 3, or esterase N (Skubitz, K.M., et al. (1989). J. Leukoc. Biol. 46(2):109-118).
Isotype	IgG2aκ
Species Reactivity	Human
Species Reactivity Note	Human.
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_001902
Gene Symbol	CTSG
Purification Method	Protein G purified
UniProt Number	P08311
Molecular Weight	28.84 kDa (prepro-form; a.a. 1-255), 26.94 kDa (pro-form; a.a. 19-255), 26.76 kDa (active form; a.a. 21-255) calculated.

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Immunocytochemistry in HL60 cells. Immunocytochemistry Analysis: A 1:200 dilution of this antibody detected Cathepsin G in HL60 human promyelocytic leukemia cells.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 μg

Transport Information

Supplemental Information

Specifications

Global Trade ITEM Number
产品目录编号	GTIN
MABT1240	04055977353600

Documentation

Anti-Cathepsin G Antibody, clone AHN-11, Ascites Free MSDS

职位
物料安全数据表 (MSDS)

Anti-Cathepsin G Antibody, clone AHN-11, Ascites Free 分析证书

标题	批号
Anti-Cathepsin G, clone AHN-11, Ascites Free - 3224556	3224556
Anti-Cathepsin G, clone AHN-11, Ascites Free - 3384847	3384847
Anti-Cathepsin G, clone AHN-11, Ascites Free - 3758625	3758625
Anti-Cathepsin G, clone AHN-11, Ascites Free - 3958299	3958299
Anti-Cathepsin G, clone AHN-11, Ascites Free - 4211811	4211811
Anti-Cathepsin G, clone AHN-11, Ascites Free -Q2694449	Q2694449

种类

Life Science Research > Antibodies and Assays > Primary Antibodies

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