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06-1372 Anti-acetyl (Lys26) phospho (Ser27) Histone H1.4 Antibody

Anti-acetyl (Lys26) phospho (Ser27) Histone H1.4 Antibody is a rabbit polyclonal antibody for detection of acetyl (Lys26) phospho (Ser27) Histone H1.4 also known as Histone H1.4, Histone H1b & has been validated in WB, DB.

Anti-acetyl (Lys26) phospho (Ser27) Histone H1.4 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histones Gene Symbol: HIST1H1E, H1F4 UniProt Number: P10412
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06-1372
100 µL  
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特卖

概述

Replacement Information

特卖

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重要规格表

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, DBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number06-1372
DescriptionAnti-acetyl (Lys26) phospho (Ser27) Histone H1.4 Antibody
Alternate Names
  • Histone H1.4
  • Histone H1b
Background InformationH1.4 is one of four variants of histone H1 (H1.2-H1.5) that is found in somatic cells. The H1 histones may function as linker proteins binding DNA via their GH domain, and enabling the wrapping of DNA around the core nucleosomal structure formed from the association of H2A, H2B, H3, and H4. In response to various stimuli, histones undergo a number of post-translational modifications (PTM) including acetylation, phoshorylation, and methylation. Previous studies have suggested that H1.4 may be phosphorylated during mitosis and interphase resulting in increased transcription of target genes by RNA polymerase I and II, and therefore this PTM on H1.4 may have implications for cell growth. H1.4 may also undergo acetylation of its N-terminal lysine residues. Often, it is the pattern of different modifications on several histone residues that alter chromatin and affect gene expression, rather than modification of single residues.
References
Product Information
FormatAffinity Purified
Control
  • Colcemid treated HeLa acid extract
PresentationPurified rabbit polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality SegmentMQ100
Applications
ApplicationAnti-acetyl (Lys26) phospho (Ser27) Histone H1.4 Antibody is a rabbit polyclonal antibody for detection of acetyl (Lys26) phospho (Ser27) Histone H1.4 also known as Histone H1.4, Histone H1b & has been validated in WB, DB.
Key Applications
  • Western Blotting
  • Dot Blot
Application NotesDot Blot Analysis: A 1:1,320 dilution from representative lot detected acetyl (Lys26) phospho (Ser27) Histone H1.4 peptides.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the N-terminus of human Histone H1.4 acetylated at Lys26 and phosphorylated at Ser27.
EpitopeN-terminus
HostRabbit
SpecificityThis antibody recognizes the N-terminus of Histone H1.4 acetylated at Lys26 and phosphorylated at Ser27.
Species Reactivity
  • Human
Species Reactivity NoteDemonstrated to react with Human. Predicted to react with Canine, Orangutan, Giant Panda, and Rhesus Macaque based on 100% sequence homology. Other homologies: Rat and Mouse (82% sequence homology).
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • HIST1H1E
  • H1F4
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~35 kDa observed. Uncharacterized bands may be observed at ~70 kDa and ~17 kDa in some acid extracts.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in colcemid treated HeLa acid extract.

Western Blot Analysis: A 1:3,000 dilution of this antibody detected acetyl (Lys26) phospho (Ser27) Histone H1.4 in 10 µg of colcemid treated HeLa acid extract.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade ITEM Number
产品目录编号 GTIN
06-1372 04053252523212

Documentation

Anti-acetyl (Lys26) phospho (Ser27) Histone H1.4 Antibody MSDS

职位

物料安全数据表 (MSDS) 

Anti-acetyl (Lys26) phospho (Ser27) Histone H1.4 Antibody 分析证书

标题批号
Anti-acetyl (Lys26) phospho (Ser27) -2780929 2780929
Anti-acetyl (Lys26) phospho (Ser27) Histone H1.4 - 2451326 2451326
Anti-acetyl (Lys26) phospho (Ser27) Histone H1.4 - 2467145 2467145
Anti-acetyl (Lys26) phospho (Ser27) Histone H1.4 - Q2032349 Q2032349

参考

参考概述公共医疗ID
Dynamics and dispensability of variant-specific histone H1 Lys-26/Ser-27 and Thr-165 post-translational modifications.
Terme, Jean-Michel, et al.
FEBS Lett., (2014)  2014

显示摘要 		24873882 24873882

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