Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

TR-1008-G Sodium Butyrate Solution

This reagent enhances the transient expression of plasmids containing the SV40 early promoter in transfected cells.

Sodium Butyrate Solution MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
View Products on Sigmaaldrich.com
TR-1008-G
1 mL  
Purchase on Sigma-Aldrich

概述

Replacement Information
Description
Catalogue NumberTR-1008-G
Trade Name
  • Specialty Media
DescriptionSodium Butyrate Solution
OverviewThis reagent enhances the transient expression of plasmids containing the SV40 early promoter in transfected cells. Available as a 500mM stock solution (1mL). A complete protocol is included with shipment.
References
Product Information
Quality SegmentMQ200
Applications
ApplicationThis reagent enhances the transient expression of plasmids containing the SV40 early promoter in transfected cells.
Application NotesSodium butyrate can act to increase the efficiency of transfection and expression for both transient and stable transfections. It increases enhancer-dependant transcription of inserts driven by the SV40 early promoter. Vectors of this type include any of the pSV and pSV2 plasmids. Treatment of transfected cells with sodium butyrate can result in: a 2-3 fold increase in the number of cells able to express the insert; a 10-30 fold increase in the number of stable transfected colonies; and cells treated with sodium butyrate at the time of transfection can be re-induced by a second treatment weeks after the initial treatment and yield a 4-7 fold increase in transcription of the SV40 early promoter driven insert.



This treatment can be used on cells transfected by the Calcium Phosphate, DEAE Dextran and electroportation method.
Biological Information
Media FormLiquid
Stem Cell Type
  • Mouse Embryonic Stem Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at 2-8°C for 2 years.
Packaging Information
Material Size1 mL
Transport Information
Supplemental Information
Specifications
Global Trade ITEM Number
产品目录编号 GTIN
TR-1008-G 04053252504877