G4259
β-Glucuronidase from Helix aspersa (garden snail)
Type HA-4
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About This Item
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-606-8
MDL number:
Specific activity:
≥300,000 units/g solid solid
type
Type HA-4
Quality Level
form
partially purified powder
specific activity
≥300,000 units/g solid solid
secondary activity
≤7,500 units/g solid sulfatase
solubility
H2O: soluble 1.90-2.10 mg/mL, clear to slightly hazy
application(s)
clinical testing
storage temp.
−20°C
Application
β-glucuronidase was used in enzymic hydrolysis of tissue homogenates for liquid chromatography-electrospray ion trap mass spectrometry (LC/MSn) analysis, to study the structures of degradation products of baicalin.
Biochem/physiol Actions
β-glucuronidase (β-GIc) is an exoglycosidase that catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.
Other Notes
One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37°C at the pH 5.0 (30 min assay).
One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C.
Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis
signalword
Danger
Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
wgk
WGK 1
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