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MABT39 Anti-Laminin alpha 5 Antibody, clone 4B12

This Anti-Laminin alpha 5 Antibody, clone 4B12 is validated for use in IH for the detection of Laminin alpha 5.

Anti-Laminin alpha 5 Antibody, clone 4B12 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: ECM Proteins Gene Symbol: LAMA5 UniProt Number: O15230
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MABT39
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIHCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABT39
      DescriptionAnti-Laminin alpha 5 Antibody, clone 4B12
      Alternate Names
      • laminin, alpha 5
      • Laminin-15 subunit alpha
      • laminin alpha-5 chain
      • Laminin-11 subunit alpha
      • laminin subunit alpha-5
      • Laminin-10 subunit alpha, Laminin α5, Lamininα5, Laminin-α5
      Background InformationLaminin alpha 5 (α5) is a member of the laminin family of extracellular matrix glycoproteins. Laminin’s are important in cell migration, differentiation and in tumor cell incursion. Research shows that laminin alpha 5 may be an early marker of sexual differentiation. In rat, the laminin alpha 5 chain seems to halt expression in the basement membrane of Sertoli cells when secretion of AMH begins but expression increases two weeks after birth when AMH secretion stops. Laminin alpha 5 shows up early during lung development in mice. It has also been linked to inflammatory cell chemotaxis and metalloproteinase activity and may play a role in creating and sustaining mucosal patterns of the mouse small intestine.
      References
      Product Information
      FormatPurified
      Control
      • Human precancerous colorectal lesion tissue
      PresentationPurified mouse monoclonal IgG1λ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality SegmentMQ100
      Applications
      ApplicationThis Anti-Laminin alpha 5 Antibody, clone 4B12 is validated for use in IH for the detection of Laminin alpha 5.
      Key Applications
      • Immunohistochemistry
      Application NotesImmunohistochemistry Analysis: A 1:100 dilution from a representative lot detected Laminin alpha 5 in human blood vessels and metastatic colorectal adenocarcinoma tissues.
      Biological Information
      ImmunogenLaminin purified from human placenta
      Clone4B12
      HostMouse
      IsotypeIgG1λ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryComponents of the extracellular matrix exert myriad effects on tissues throughout the body. In particular, the laminins, a family of heterotrimeric extracellular glycoproteins, affect tissue development and integrity in such diverse organs as the kidney, lung, skin, and nervous system. It is thought that laminins mediate the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Laminins function as heterotrimeric complexes of alpha, beta, and gamma chains, with each chain type representing a different subfamily of proteins. The protein encoded by this gene belongs to the alpha subfamily of laminin chains and is a major component of basement membranes. Two transcript variants encoding different isoforms have been found for this gene, but the full-length nature of one of them has not been determined. [provided by RefSeq].
      Gene Symbol
      • LAMA5
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

      SUBUNIT STRUCTURE: Laminin is a complex glycoprotein, consisting of three different polypeptide chains (alpha, beta, gamma), which are bound to each other by disulfide bonds into a cross-shaped molecule comprising one long and three short arms with globules at each end. Alpha-5 is a subunit of laminin-10 (laminin-511), laminin-11 (laminin-521) and laminin-15 (laminin-523).

      SUBCELLULAR LOCATION: Secreted › extracellular space › extracellular matrix › basement membrane. Note: Major component.

      TISSUE SPECIFICTY: Expressed in heart, lung, kidney, skeletal muscle, pancreas, retina and placenta. Little or no expression in brain and liver.

      DOMAIN: Domain G is globular and is part of the major cell-binding site located in the long arm of the laminin heterotrimer.

      SEQUENCE SIMILARITIES: Contains 22 laminin EGF-like domains.

      Contains 5 laminin G-like domains.

      Contains 1 laminin IV type A domain.

      Contains 1 laminin N-terminal domain.

      SEQUENCE CAUTION: The sequence CAC22310.1 differs from that shown. Reason: Erroneous gene model prediction.
      Molecular Weight400 kDa calculated
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in human precancerous colorectal lesion tissue.

      Immunohistochemistry Analysis: A 1:100 dilution of this antibody detected Laminin alpha 5 in human precancerous colorectal lesion tissue.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade ITEM Number
      Catalogue Number GTIN
      MABT39 04053252646126

      Documentation

      Anti-Laminin alpha 5 Antibody, clone 4B12 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Laminin alpha 5 Antibody, clone 4B12 Certificates of Analysis

      TitleLot Number
      Anti-Laminin alpha 5, clone 4B12 - 2147127 2147127
      Anti-Laminin alpha 5, clone 4B12 - 2395813 2395813
      Anti-Laminin alpha 5, clone 4B12 - 2470509 2470509
      Anti-Laminin alpha 5, clone 4B12 - 2207228 2207228
      Anti-Laminin alpha 5, clone 4B12 - 2297287 2297287
      Anti-Laminin alpha 5, clone 4B12 - 3204240 3204240
      Anti-Laminin alpha 5, clone 4B12 - 3483441 3483441
      Anti-Laminin alpha 5, clone 4B12 - 3714396 3714396
      Anti-Laminin alpha 5, clone 4B12 - 4215443 4215443
      Anti-Laminin alpha 5, clone 4B12 - NRG1843064 NRG1843064
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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies