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Merck

N1531

Denaturation Solution

for Northern and Southern blotting

동의어(들):

Denaturation Solution For Blotting Technique, Denaturation Solution for Neutral Southern Transfer

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NACRES:
NA.25
UNSPSC Code:
12161700
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도움 문의


grade

Molecular Biology

Quality Segment

form

solution

storage temp.

room temp

General description

Sigma′s denaturing solution is a strong base that denatures double-stranded DNA so that it can be efficiently transferred to nylon membrane and subsequently hybridized to labeled probe. When used with a depurination solution, treatment results in the hydrolysis of the phosphodiester backbone at the sites of depurination, allowing for more efficient transfer of large DNA in neutral Southern transfers.

Application

Denaturation Solution is suitable for denaturing dsDNA before the transfer to membranes during Northern and Southern blotting procedures.

Other Notes

0.5 N NaOH, 1.5 M NaCl
The denaturing solutions is composed of 0.5N NaOH, and 1.5M NaCl.


pictograms

Corrosion

signalword

Danger

hcodes

Hazard Classifications

Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1B

저장 등급

8B - Non-combustible corrosive hazardous materials

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



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