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MABE461 Anti-CRTC1 Antibody/TORC-1, clone 5F3.1

This Anti-CRTC1 Antibody/TORC-1, clone 5F3.1 is validated for use in Western Blotting, Immunohistochemistry, Immunoprecipitation for the detection of CRTC1.

Anti-CRTC1 Antibody/TORC-1, clone 5F3.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: CRTC1, KIAA0616, MECT1, TORC1, WAMTP1 UniProt Number: Q6UUV9
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MABE461
100 μg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, H, RWB, IHC, IPMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE461
      DescriptionAnti-CRTC1 Antibody/TORC-1, clone 5F3.1
      Alternate Names
      • CREB-regulated transcription coactivator 1
      • Mucoepidermoid carcinoma translocated protein 1
      • Transducer of regulated cAMP response element-binding protein 1
      • TORC-1
      • Transducer of CREB protein 1
      • CRTC1/TORC-1
      Background InformationCREB-regulated transcription coactivator 1 (UniProt Q6UUV9; also known as Mucoepidermoid carcinoma translocated protein 1, TORC-1, Transducer of CREB protein 1, Transducer of regulated cAMP response element-binding protein 1) is encoded by the CRTC1 (also known as KIAA0616, MECT1, TORC1, WAMTP1) gene (Gene ID 23373) in human. CRTC1 belongs to a family of glycosylated Ser/Thr-rich phosphoproteins that bind the basic leucine zipper (bZIP) region of Ca2+/cAMP-responsive element-binding protein (CREB) and function as CREB coactivators. CRTCs participate in CREB-mediated transcriptions of both canonical cellular cAMP response elements (CREs) and the non-canonical viral CREs. CRTC1, specifically, is involved in CREB-mediated transcription of various neuronal immediate early genes (IEGs) as well as CREB-dependent hepatitis B virus (HBV) replication.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality SegmentMQ100
      Applications
      ApplicationThis Anti-CRTC1 Antibody/TORC-1, clone 5F3.1 is validated for use in Western Blotting, Immunohistochemistry, Immunoprecipitation for the detection of CRTC1.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Immunoprecipitation
      Application NotesWestern Blotting Analysis: 1.0 µg/mL from a representative lot detected CRTC1/TORC-1 in 10 µg of mouse hypothalamus tissue lysate.
      Immunohistochemistry Analysis: A 1:50-1,000 dilution from a representative lot detected CRTC1/TORC-1 in human cerebral cortex, rat cerebral cortex, human cerebellum, and rat cerebellum tissue.
      Immunoprecipitation Analysis: A representative lot immunoprecipitated endogenous CRTC1/TORC-1 from mouse hippocampus tissue lysates, as well as exogenously expressed murine CRTC1/TORC-1, but not CRTC2/TORC-2, from lysates of transfected HEK293 cells (Courtesy of Dr. Carlos A. Saura , Universitat Autònoma de Barcelona, Spain).
      Western Blotting Analysis: A representative lot detected endogenous CRTC1/TORC-1 in human, mouse, and rat hippocampus tissue lysates, as well as exogenously expressed murine CRTC1/TORC-1 in lysates from transfected HEK293 cells (Courtesy of Dr. Carlos A. Saura , Universitat Autònoma de Barcelona, Spain).
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to human CRTC1/TORC-1 sequence within the N-terminal half..
      EpitopeInternal (N-terminal half)
      Clone5F3.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityExpected to react with all three human spliced isoforms
      IsotypeIgG2aκ
      Species Reactivity
      • Mouse
      • Human
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CRTC1
      • KIAA0616
      • MECT1
      • TORC1
      • WAMTP1
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~68 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse cerebellum tissue lysate.

      Western Blotting Analysis: 1.0 µg/mL of this antibody detected CRTC1/TORC-1 in 10 µg of mouse cerebellum tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade ITEM Number
      Catalogue Number GTIN
      MABE461 04055977277364

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies