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ABE212 Anti-Set1A Antibody

Use Anti-Set1A Antibody (Rabbit Polyclonal Antibody) validated in WB to detect Set1A also known as SET domain containing 1A, Set1/Ash2 histone methyltransferase complex subunit SET1.

Anti-Set1A Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histone Modifying Proteins Gene Symbol: hSET1A, SET1, SET1A, SETD1A, Set1, Set1A UniProt Number: O15047
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ABE212
100 µL  
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      Overview

      Replacement Information

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, ChIPRbSerumPolyclonal Antibody
      Description
      Catalogue NumberABE212
      DescriptionAnti-Set1A Antibody
      Alternate Names
      • SET domain containing 1A
      • Lysine N-methyltransferase 2F
      • histone-lysine N-methyltransferase SETD1A
      • Set1/Ash2 histone methyltransferase complex subunit SET1
      • SET domain-containing protein 1A
      Background InformationSet1A is a histone methyltransferase (HMT) that is part of the SET1 HMT complex. This protein specifically methylates lysine 4 of histone H3 if the adjacent lysine at position 9 is also unmethylated. Studies demonstrate that Set1A and Set1B localize to largely non-overlapping regions of the chromosome. This suggests Set1A and Set1B bind to unique sets of target genes and that each protien makes non-redundant contributions to the epigenetic control of chromatin structure and gene expression.
      References
      Product Information
      FormatSerum
      Control
      • PC3 cell lysate
      PresentationRabbit polyclonal serum containing 0.05% sodium azide.
      Quality SegmentMQ100
      Applications
      ApplicationUse Anti-Set1A Antibody (Rabbit Polyclonal Antibody) validated in WB to detect Set1A also known as SET domain containing 1A, Set1/Ash2 histone methyltransferase complex subunit SET1.
      Key Applications
      • Western Blotting
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesChromatin Immunoprecipitation (ChIP):
      A representative lot of this antibody was reported to work in ChIP. See Lee, J.H., et al.(2008). Mol Cell Biol. 28(2):609–618.

      Immunocytochemistry:
      A representative lot of this antibody was reported to work in immunocytochemistry. See . Lee, J.H., et al. (2007). J Biol Chem. 282(18):13419-13428.
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to human Set1A.
      HostRabbit
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummarySET1A is a component of a histone methyltransferase (HMT) complex that produces mono-, di-, and trimethylated histone H3 at Lys4. The complex is the analog of the S. cerevisiae Set1/COMPASS complex (Lee and Skalnik, 2005 [PubMed 16253997]).
      Gene Symbol
      • hSET1A
      • SET1
      • SET1A
      • SETD1A
      • Set1
      • Set1A
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: Histone methyltransferase that specifically methylates 'Lys-4' of histone H3, when part of the SET1 histone methyltransferase (HMT) complex, but not if the neighboring 'Lys-9' residue is already methylated. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. The non-overalpping localization with SETD1B suggests that SETD1A and SETD1B make non-redundant contributions to the epigenetic control of chromatin structure and gene expression.

      CATALYTIC ACTIVITY: S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].

      SUBUNIT STRUCTURE: Interacts with HCFC1. Component of the SET1 complex, at least composed of the catalytic subunit (SETD1A or SETD1B), WDR5, WDR82, RBBP5, ASH2/ASH2L and CXXC1/CFP1.

      SUBCELLULAR LOCATION: Nucleus speckle. Chromosome. Note: Localizes to a largely non-overlapping set of euchromatic nuclear speckles with SETD1B, suggesting that SETD1A and SETD1B each bind to a unique set of target genes.

      SEQUENCE SIMILARITIES: Contains 1 post-SET domain.

      Contains 1 RRM (RNA recognition motif) domain.

      Contains 1 SET domain.

      SEQUENCE CAUTION: The sequence AAH27450.1 differs from that shown. Reason: Erroneous initiation.

      The sequence AAH35795.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.

      The sequence BAA20797.2 differs from that shown. Reason: Erroneous initiation.
      Molecular Weight ~186 kDa observed. A cross-reacting band appears at ~45 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in PC3 cell lysate.

      Western Blot Analysis: 1:1,000 dilution of this antibody detected Set1A on 10 µg of PC3 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade ITEM Number
      Catalogue Number GTIN
      ABE212 04053252414596