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MABN24 Anti-pan-Shank Antibody, clone N23B/49

Detect pan-Shank using this Anti-pan-Shank Antibody, clone N23B/49 validated for use in WB, IH(P).

Anti-pan-Shank Antibody, clone N23B/49 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Synapse & Synaptic Biology Gene Symbol: SHANK2, CORTBP1, ProSAP1, CTTNBP1, SPANK-3, SHANK, PROSAP1, CortBP1, Shank2 UniProt Number: Q9QX74
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MABN24
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, M, HWB, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN24
      DescriptionAnti-pan-Shank Antibody, clone N23B/49
      Alternate Names
      • SH3 and multiple ankyrin repeat domains 2
      • SH3 and multiple ankyrin repeat domains protein 2
      • proline-rich synapse associated protein 1
      • cortactin SH3 domain-binding protein
      • Cortactin-binding protein 1
      • Proline-rich synapse-associated protein 1
      • cortactin binding protein 1
      • GKAP/SAPAP interacting protein
      Background InformationShank1, Shank2, and Shank3 make up a family of proteins that may function as molecular scaffolds in the postsynaptic density (PSD). Shank contains multiple domains for protein-protein interaction including ankyrin repeats, an SH3 domain, a PSD-95/Dlg/ZO-1 domain, a sterile α motif domain, and a proline-rich region. Alternative splicing in the Shank family may be a mechanism that regulates the molecular structure of Shank and the spectrum of Shank-interacting proteins in the PSDs of adult and developing brain.
      References
      Product Information
      FormatPurified
      Control
      • Rat brain membrane tissue lysate
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality SegmentMQ100
      Applications
      ApplicationDetect pan-Shank using this Anti-pan-Shank Antibody, clone N23B/49 validated for use in WB, IH(P).
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: 1:500 dilution from a previous lot detected Shank in rat cerebellum tissue.

      Western Blot Analysis: A previous lot of this antibody detected Shank in extracts of COS-1 cells transiently transfected with Shank1, Shank2 or Shank3 plasmids. Courtesy of James Trimmer, UC Davis/NIH NeuroMab Facility.
      Biological Information
      ImmunogenRecombinant protein consisting of SH3/PDZ domain of rat Shank2.
      CloneN23B/49
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Rat
      • Mouse
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SHANK2
      • CORTBP1
      • ProSAP1
      • CTTNBP1
      • SPANK-3
      • SHANK
      • PROSAP1
      • CortBP1
      • Shank2
      Purification MethodProtein G
      UniProt Number
      UniProt SummaryFUNCTION: Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors, and the actin-based cytoskeleton. May play a role in the structural and functional organization of the dendritic spine and synaptic junction.

      SUBUNIT STRUCTURE: Interacts with DNM2 and BAIAP2. Interacts with GRID2 (By similarity). Interacts with CCTN/cortactin SH3 domain, DLGAP1/GKAP and alpha-latrotoxin receptor 1. Is part of a complex with DLG4/PSD-95 and DLGAP1/GKAP. Interacts with SLC9A3 and PLCB3. Interacts with DBNL. Interacts with CFTR.

      SUBCELLULAR LOCATION: Cytoplasm. Cell junction › synapse. Cell junction › synapse › postsynaptic cell membrane › postsynaptic density. Cell projection › growth cone. Note: Cytoplasm, postsynaptic density of neuronal cells. Colocalizes with cortactin in growth cones in differentiating hippocampal neurons.

      TISSUE SPECIFICTY: All isoforms except isoform 7 are expressed predominantly in brain, with highest levels in olfactory bulb, cerebral cortex, cerebellum, central gray matter and hippocampus. Moderate levels of expression are seen in the caudate putamen, thalamic nuclei and brain stem. In cerebellum primarily expressed in Purkinje cells. Isoform 7 is not expressed in brain but expressed in liver, cholangiocytes and thymus. Isoform 7 is present in pancreas, colonic mucosa and thymocytes (at protein level).

      DEVELOPMENTAL STAGE: Expressed during early postnatal brain development, especially in the caudate putamen and thalamic nuclei. Expression in the cerebral cortex, the hippocampus and the cerebellum is moderate to high at P5 and shows a stable expression throughout development. Isoforms 1, 2, 4 and 6 are predominantly expressed in cerebellum up to the age of approximately 3 weeks. Isoform 1 expression decreases during development of cortex but slightly increases in cerebellum.

      DOMAIN: The PDZ domain is required for interaction with GRID2, PLCB3, SLC9A3 and CFTR.

      SEQUENCE SIMILARIITIES: Belongs to the SHANK family.

      Contains 1 PDZ (DHR) domain.

      Contains 1 SAM (sterile alpha motif) domain.

      Contains 1 SH3 domain.
      Molecular Weight~160 kDa observed. Other isoforms may be observed in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in rat brain membrane tissue lysate.

      Western Blot Analysis: 2 µg/mL of this antibody detected Shank on 10 µg of rat brain membrane tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade ITEM Number
      Catalogue Number GTIN
      MABN24 04053252523830

      Documentation

      Anti-pan-Shank Antibody, clone N23B/49 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-pan-Shank Antibody, clone N23B/49 Certificates of Analysis

      TitleLot Number
      Anti-pan-Shank, clone N23B/49 - 2445048 2445048
      Anti-pan-Shank, clone N23B/49 - 2043216 2043216
      Anti-pan-Shank, clone N23B/49 - 2192072 2192072
      Anti-pan-Shank, clone N23B/49 - 2283569 2283569
      Anti-pan-Shank, clone N23B/49 - 3328966 3328966
      Anti-pan-Shank, clone N23B/49 - 3468322 3468322
      Anti-pan-Shank, clone N23B/49 - 3574547 3574547
      Anti-pan-Shank, clone N23B/49 - 3886038 3886038
      Anti-pan-Shank, clone N23B/49 - 4132012 4132012
      Anti-pan-Shank, clone N23B/49 - 4231222 4231222
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