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07-2126 Anti-phospho-FOXO1 Antibody (Thr24)

Use Anti-phospho-FOXO1 (Thr24) Antibody (Rabbit Polyclonal Antibody) validated in WB to detect phospho-FOXO1 (Thr24) also known as Forkhead box protein O1.

Anti-phospho-FOXO1 Antibody (Thr24) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Transcription Factors Gene Symbol: FOXO1, FOXO1A, FKHR UniProt Number: Q12778
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07-2126
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-2126
      DescriptionAnti-phospho-FOXO1 Antibody (Thr24)
      Alternate Names
      • Forkhead box protein O1, Forkhead box protein O1A, Forkhead in rhabdomyosarcoma
      Background InformationForkhead box O (FOXO) transcription factors play an important role in modulating metabolic functions and cell proliferation. Given the relatively high expression of FOXO1 in insulin-responsive tissues, this transcription factor is highly poised to regulate energy metabolism. When nutrient and insulin levels are low, FOXO1 promotes expression of gluconeogenic enzymes. Conversely, in the fed state, insulin levels rise and stimulate uptake of glucose primarily into skeletal muscle and other organs, including adipose tissue. Under certain pathophysiologic conditions, including insulin resistance, negative signaling to FOXO1 is compromised. Increased cell proliferation occurs when FOXO1 is inactivated when phosphorylated by Akt at Thr24.
      References
      Product Information
      FormatAffinity Purified
      Control
      • HEK293 cell lysate untreated and BMP2-treated
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality SegmentMQ100
      Applications
      ApplicationUse Anti-phospho-FOXO1 (Thr24) Antibody (Rabbit Polyclonal Antibody) validated in WB to detect phospho-FOXO1 (Thr24) also known as Forkhead box protein O1.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human FOXO1 phosphorylated at Thr24.
      EpitopePhosphorylated Thr24
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes FOXO1 phosphorylated at Thr24.
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • FOXO1
      • FOXO1A
      • FKHR
      Modifications
      • Phosphorylation
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFunction: Transcription factor which acts as a regulator of cell responses to oxidative stress. In the presence of KIRT1, mediates down-regulation of cyclin D1 and up-regulation of CDKN1B levels which are required for cell transition from proliferative growth to quiescence.

      Subunit structure: Interacts with LRPPRC. Interacts with SIRT1 and this interaction requires the presence of KRIT1.

      Subcellular location: Cytoplasm. Nucleus. Note: Shuttles between cytoplasm and nucleus.

      Tissue specificity: Ubiquitous.

      Induction: Expression is regulated by KRIT1.

      Post-translational modification: Phosphorylated by AKT1; insulin-induced. IGF1 rapidly induces phosphorylation of Ser-256, Thr-24, and Ser-319. Phosphorylation of Ser-256 decreases DNA-binding activity and promotes the phosphorylation of Thr-24, and Ser-319, permitting phosphorylation of Ser-322 and Ser-325, probably by CK1, leading to nuclear exclusion and loss of function. Phosphorylation of Ser-329 is independent of IGF1 and leads to reduced function. Phosphorylated upon DNA damage, probably by ATM or ATR.

      Involvement in disease: Defects in FOXO1 are a cause of rhabdomyosarcoma type 2 (RMS2) [MIM:268220]. It is a form of rhabdomyosarcoma, a highly malignant tumor of striated muscle derived from primitive mesenchimal cells and exhibiting differentiation along rhabdomyoblastic lines. Rhabdomyosarcoma is one of the most frequently occurring soft tissue sarcomas and the most common in children. It occurs in four forms: alveolar, pleomorphic, embryonal and botryoidal rhabdomyosarcomas. Note=Chromosomal aberrations involving FOXO1 are found in rhabdomyosarcoma. Translocation (2;13)(q35;q14) with PAX3; translocation t(1;13)(p36;q14) with PAX7. The resulting protein is a transcriptional activator.

      Sequence similarities: Contains 1 fork-head DNA-binding domain.
      Molecular Weight~78 kDa observed.
      Calculated molecular weight is 70 kDa. FOXO1 has been shown to migrate to ~78 kDa in western blots (Kamei, Y., et al. (2004). JBC. 279(39):41114-41123).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HEK293 cell lysate untreated and BMP2-treated.

      Western Blot Analysis: 2 µg/mL of this antibody detected phospho-FOXO1 (Thr24) in HEK293 cell lysate untreated and BMP2-treated.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade ITEM Number
      Catalogue Number GTIN
      07-2126 04053252633287

      Documentation

      Anti-phospho-FOXO1 Antibody (Thr24) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-phospho-FOXO1 Antibody (Thr24) Certificates of Analysis

      TitleLot Number
      Anti-phospho-FOXO1 (Thr24) - 2006947 2006947
      Anti-phospho-FOXO1 (Thr24) - 2196090 2196090
      Anti-phospho-FOXO1 (Thr24) - 2514213 2514213
      Anti-phospho-FOXO1 (Thr24) - 3026970 3026970
      Anti-phospho-FOXO1 (Thr24) - 3288738 3288738
      Anti-phospho-FOXO1 (Thr24) - 3407325 3407325
      Anti-phospho-FOXO1 (Thr24) - 4149314 4149314
      Anti-phospho-FOXO1 (Thr24) - 4279399 4279399
      Anti-phospho-FOXO1 (Thr24) - NRG1912576 NRG1912576
      Anti-phospho-FOXO1 (Thr24) -2753836 2753836
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