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09-203 Anti-phospho-JAK2 (Tyr868) Antibody

Anti-phospho-JAK2 (Tyr868) Antibody detects level of phospho-JAK2 (Tyr868) & has been published & validated for use in 0.

Anti-phospho-JAK2 (Tyr868) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Immunological Signaling UniProt Number: O60674
09-203
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number09-203
      DescriptionAnti-phospho-JAK2 (Tyr868) Antibody
      Background InformationJAK2 (Janus Kinase 2) belongs to the emerging family of non-receptor Janus tyrosine kinases, which regulate a spectrum of cellular functions downstream of activated cytokine receptors in the lympho-hematopoietic system. Immunological stimuli, such as interferons and cytokines, induce recruitment of Stat transcription factors to cytokine receptor-associated JAK2. JAK2 then phosphorylates proximal Stat factors, which subsequently dimerize, translocate to the nucleus and bind to CIS elements upstream of target gene promoters to regulate transcription. The canonical JAK/Stat pathway is integral to maintaining a normal immune system by stimulating proliferation, differentiation, survival and host resistance to pathogens. Altering JAK/Stat signaling to reduce cytokine induced pro-inflammatory responses represents an attractive target for anti-inflammatory therapies. Within the JAK2 kinase domain, there is a region that has considerable sequence homology to the regulatory region of the insulin receptor.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Applications
      ApplicationAnti-phospho-JAK2 (Tyr868) Antibody detects level of phospho-JAK2 (Tyr868) & has been published & validated for use in 0.
      Key Applications
      • Western Blotting
      Application NotesWestern Blot (SNAP ID) Analysis: 1:50 dilution from a previous lot detected phospho-JAK2 on 10 µg of cell lysates of HEK293T stimulated with SH2b1beta.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the protein kinase 2 domain of human phospho-JAK2.
      EpitopeProtein kinase 2 domain
      HostRabbit
      SpecificityThis antibody recognizes the protein kinase 2 domain of JAK2.
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteDemonstrated to react with human and mouse. Predicted to react with rat, equine, opossum, bovine, chimpanzee, porcine, ape, and platypus based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      UniProt Number
      UniProt SummaryFUNCTION:Plays a role in leptin signaling and control of body weight By similarity. Tyrosine kinase of the non-receptor type, involved in interleukin-3 and probably interleukin-23 signal transduction.

      CATALYTIC ACTIVITY:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

      SUBUNIT STRUCTURE: Interacts with SIRPA and SH2B1 By similarity. Interacts with IL23R, SKB1 and STAM2.

      SUBCELLULAR LOCATION: Endomembrane system; Peripheral membrane protein By similarity. Note: Wholly intracellular, possibly membrane associated By similarity.

      TISSUE SPECIFICITY: Expressed in blood, bone marrow and lymph node.

      DOMAIN: Possesses two phosphotransferase domains. The second one probably contains the catalytic domain By similarity, while the presence of slight differences suggest a different role for domain 1.

      PTM:Leptin promotes phosphorylation on tyrosine residues, including phosphorylation on Tyr-813 By similarity.

      INVOLVEMENT IN DISEASE: Chromosomal aberrations involving JAK2 are found in both chronic and acute forms of eosinophilic, lymphoblastic and myeloid leukemia. Translocation t(8;9)(p22;p24) with PCM1 links the protein kinase domain of JAK2 to the major portion of PCM1. Translocation t(9;12)(p24;p13) with ETV6.Defects in JAK2 are a cause of susceptibility to Budd-Chiari syndrome [MIM:600880]. Budd-Chiari syndrome is a spectrum of disease states, including anatomic abnormalities and hypercoagulable disorders, resulting in hepatic venous outflow occlusion. Clinical manifestations observed in the majority of patients include hepatomegaly, right upper quadrant pain, and abdominal ascites.

      Defects in JAK2 are associated with polycythemia vera (PV) [MIM:263300]. PV, the most common form of primary polycythemia, is caused by somatic mutation in a single hematopoietic stem cell leading to clonal hematopoiesis. PV is a myeloproliferative disorder characterized predominantly by erythroid hyperplasia, but also by myeloid leukocytosis, thrombocytosis, and splenomegaly. Familial cases of PV are very rare and usually manifest in elderly patients.

      Defects in JAK2 gene may be a cause of essential thrombocythemia (ET) [MIM:187950]. ET is characterized by elevated platelet levels due to sustained proliferation of megakaryocytes, and frequently lead to thrombotic and haemorrhagic complications.

      Defects in JAK2 are associated with familial myelofibrosis [MIM:254450]. Myelofibrosis with myeloid metaplasia is a myeloproliferative disease with annual incidence of 0.5-1.5 cases per 100,000 individuals and age at diagnosis around 60 (an increased prevalence is noted in Ashkenazi Jews). Clinical manifestations depend on the type of blood cell affected and may include anemia, pallor, splenomegaly, hypermetabolic state, petechiae, ecchymosis, bleeding, lymphadenopathy, hepatomegaly, portal hypertension.

      Defects in JAK2 are a cause of acute myelogenous leukemia (AML) [MIM:601626]. AML is a malignant disease in which hematopoietic precursors are arrested in an early stage of development.

      SEQUENCE SIMILARITIES:Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.

      Contains 1 FERM domain.

      Contains 1 protein kinase domain.

      Contains 1 SH2 domain. ...
      Molecular Weight~125 kDa Observed UniProtKB/Swiss-Prot O60674 (JAK2_HUMAN) lists a mass of 130,674 Da.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in cell lysates of HEK293T stimulated with SH2b1beta.

      Western Blot Analysis: 1:500 dilution of this antibody detected phospho-JAK2 on 10 µg of cell lysate of HEK293T stimulated with SH2b1beta.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade ITEM Number
      Catalogue Number GTIN
      09-203 04053252746659

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies