Pharmaceutical Analysis: 2-Oxoacids


Column LiChroCART® 250-4 LiChrospher® 100 RP-18, 5 μm
Pharmaceutical analysis: 2-Oxoacids
Mobile phase

Methanol/Water/Acetonitrile 35/45/20 (v/v/v)
Flow rate 1.0 mL/min 
Detection Fluorescence Ex 350 nm, Em 410 nm
Temperature room temperature
Injection volume 100 μL
Sample

1. Pyruvate
2. -
3. 2-Oxobutyric acid
4. 2-Oxoisovaleric acid (from valine)
5. 2-Oxoisocaproic acid (from leucine)
6. 2-Oxocaproic acid (internal standard)