PCR去除过滤板
Recommended Products
概述
规格
订货信息
Documentation
参考
| 参考概述 | 应用 |
|---|---|
| PCR Amplification on a Microarray of Gel-Immobilized Oligonucleotides: Detection of Bacterial Toxin- and Drug-Resistant Genes and Their Mutations Strizhkov, B.N. Drobyshev, A.L. Mikhailovich, V.M. Mirzabekov, A.D. BioTechniques 29:844-857 2000 | |
| A Concise Guide to cDNA Microarray Analysis P. Hegde, R. Qi, K. Abernathy, C. Gay, S. Dharap, R. Gaspard, J.E. Hughes, E. Snesrud, N. Lee, and J. Quackenbush BioTechniques Vol. 29, No. 3: pp 548-562 (Sep 2000) 2000 | PCR |
| A specific and sensitive PCR assay suitable for large-scale detection of toxigenic Pasteurella multocida in nasal and tonsillar swabs specimens of pigs Kamp, E.M. etal., J.Vet Diagn Invest 8:, 304-309 J.Vet Diagn Invest 8:, 304-309 1996 | Nucleic Acid Purification and Concentration |
| Use of MultiScreen Plates for the Preparation of Bacterial DNA Suitable for PCR Reek, F.H., Smits, M.A., Kamp, E. M., Smith, H.E. BioTechniques. 19: 2, 282 – 285 1995 | Nucleic Acid Purification and Concentration |
问与答
| 问题 | 回答 |
|---|---|
| Can the Montage PCR-96 Kit be used in a procedure employing centrifugal rather than vacuum filtration? | Use of the Montage PCR-96 Kit in a centrifugal protocol is not recommended. |
| Can the MultiScreen plates included with the Montage kit be used with a vacuum manifold from a vendor other than Millipore? | Yes, but only if proper underdrain support grid is employed. Please contact your local Millipore technical service organization for specific information on the availability of the "underdrain supports" for non-Millipore vacuum manifolds. |
| Is a wash step required following the initial filtration with the Montage PCR-96 Cleanup Kit? | For many applications, a wash step is not necessary. If you need wash the purified PCR products, use the nuclease free water included in the PCR cleanup kit. |
| Should I use water or Tris buffer to resuspend the final PCR products? | Either solution may be used with the Montage PCR-96 Kit. A user may prefer one of these depending on the downstream application. |
| What are the minimum and maximum loading capacities for the MultiScreen plates used with the Montage PCR-96 Kit? | There is a 1 ug minimum capacity and no maximum limit. |
| What is filtration time for the the Montage PCR-96 Kit? | Filtration time is approximately 10 min, depending on volume of sample. |
| Is the Montage PCR-96 kit capable of eliminating Triton or other detergents from the buffers used in PCR reactions? | It is difficult to completely remove Triton. We recommend the use of a PCR buffer that is Triton-free. Alternatively, one can use MultiScreen FB plates with TN063 protocol. |
| Can the MultiScreen-PCR plate be used in a centrifugal mode? | The MultiScreen-PCR plates are designed for use in the vacuum mode and use in the centrifugal mode is not recommended. |
| Can the MultiScreen-PCR plate be used with another manufacturers vacuum manifold? | No, the plate needs the support grid of the Millipore vacuum manifold. |
| How can I increase the recovery of my 100-200 BP PCR fragment in the PCR "clean-up" application? | Millipore recommends use of the MultiScreen FB plate for "clean-up" of PCR fragnents of this size, especially with low concentrations of DNA fragments. |
相关产品和应用
相关产品，按下列排序： Application Facete
| Protein Purification |
| Sample Preparation |
| Dialysis and Filtration |
相关产品，按下列排序： Brand Facete
| MultiScreen™ |
种类
| Life Science Research > Cell Culture and Systems > Cell Culture Flasks, Plates, & Slides > Multiwell Plates |
我们的PCR过滤板是大呑吐量PCR纯化的快速、可自动操作的解决方案。MultiScreen® PCR板有96-和384-孔两种型号，µ96孔板用于小体积样品。这些过滤板使用的是粒径排除滤膜和真空过滤方式，提供了只需一个步骤的试验方案，而且结果出色。不需离心或沉淀。全部样品均从板顶回收，这增强了与液体处置系统的兼容性。MultiScreen® PCRµ96和MultiScreen® PCR384过滤板回收率高，微型化反应最小。工作体积<150 µL，节省了试剂和费用。对于体积>150 µL的情况，请使用MultiScreen® PCR96 96孔过滤板。
特点及优点
- 高纯度和高回收率
- 不需离心或沉淀
- 处理速度快，可自动操作
- 对于小片段（1-150 bp），建议使用MultiScreen® PCRµ96过滤板
实验方案
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| 1. | 装载PCR反应物。 |
| 2. | 用Millipore真空多联装置过滤5–10分钟，直到板孔都干了为止。 |
| 3. | 向每个板孔添加水或缓冲液。用摇动或移液的方式搅拌。用吸入方式回收已纯化的的样品。 |
选择指南
| 规格 | |||||
| 产品名称 | 反应物体积 | 样品 个数 |
操作 模式 |
建议的 应用 |
特点 |
| MultiScreen® PCRµ96 过滤板 | 20 – 150 µL | 96 | 真空 | 测序 基因分型 微阵列 |
• 高度浓缩的最终样品 • 反应微型化 |
| MultiScreen® PCR96过滤板 | 150–300 µL | 96 | 真空 | 微阵列 | • 单位体积能力大 |
| MultiScreen® PCR384 过滤板 | 20–100 µL | 384 | 真空 | 测序 基因分型 微阵列 |
• 高度浓缩的最终样品 • 反应微型化 • 超大呑吐量 |
回收率
| 产品名称 | 137 bp PCR片断的回收率* | 301 bp PCR片断的回收率* | 657 bp PCR片断的回收率* | 1159 bp PCR片断的回收率* | 底物去除率%（20种碱），100 µL体积 |
| MultiScreen® PCRµ96 过滤板 | + | ++ | ++ | ++ | 99.8% |
| MultiScreen® PCR96过滤板 | -- | + | ++ | ++ | 98.7% |
| MultiScreen® PCR384 过滤板 | + | ++ | ++ | ++ | 99.5% |
| -- 不建议使用 | |||||
| + 回收率好 | |||||
| ++ 回收率最好 | |||||
| *结果将随起始浓度、样品量和所使用的缓冲液的不同而不同。数据是用下列浓度获得： 137 bp: 10 ng/µL 301 bp: 30 ng/µL 657 bp: 55 ng/µL 1159 bp: 71 ng/µL |
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性能
使用 MultiScreen PCR96 滤膜板，重现性高
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琼脂糖凝胶显示回收 500 bp PCR 产物的重现性，使用 MultiScreen PCR96 滤膜板。
长测序图谱
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使用 MultiScreen PCR96 滤膜板纯化后的 301bp PCR 产物的典型电泳谱图 (Thermo Sequenase® II dye terminator)。
适用于芯片
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一个具有 19,299 个位点的芯片的放大部分（图片由 Dr. John Quakenbush 授权复制，The Institute for Genomic Research）。 使用经 MultiScreen PCR 产品纯化后的 PCR 产物，可生产出高质量的芯片。 参考资料：Hegde, P., R. Qi, K. Abernathy, C. Gay, S. Dhaarp, R. Gaspard, J.E. Hughes, E. Snesrud, N. Lee and J. Quakenbush. 2000. A concise guide to cDNA microarray analysis. BioTechniques 29: 548–562.
应用
PCR去除，测序，基因分型，微阵列，RNAi纯化，微阵列纯化，基因组DNA浓缩