I1646
IL-3 human
≥97% (HPLC and SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
Synonym(s):
Interleukin-3 human, hIL-3, IL-3
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About This Item
Biological source:
human
Recombinant:
expressed in E. coli
Assay:
≥97% (HPLC and SDS-PAGE)
Form:
lyophilized powder
Mol wt:
15 kDa
Impurities:
≤0.1 EU/μg
Product Name
Interleukin-3 human, IL-3, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
biological source
human
Quality Segment
recombinant
expressed in E. coli
assay
≥97% (HPLC and SDS-PAGE)
form
lyophilized powder
potency
≤1.0 ng/mL ED50/EC50
quality
endotoxin tested
mol wt
15 kDa
packaging
pkg of 10 and 50 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
≤0.1 EU/μg
UniProt accession no.
storage temp.
−20°C
Biochem/physiol Actions
Interleukin-3 (IL-3) is a multifunctional protein, originally called colony forming unit-stimulating activity (CFU-SA), and is produced by activated T lymphocytes. IL-3 supports the formation of multilineage colonies in the early development of multipotent hematopoietic progenitor cells. IL-3 has been shown to induce colony formation of macrophages, neutrophils, mast cells, and megakaryocytes from agar-suspended bone marrow cells. IL-3 also interacts with IL-2 to stimulate growth of T lymphocytes and to induce IgG secretion from activated B cells. Other synonyms for IL-3 include pan-specific hemopoietin, multicolony stimulating factor, mast cell growth factor, burst promoting activity, histamine-producing cell stimulating factor, P cell stimulating factor, and WEHI-3 factor.
Interleukin-3 is a multifunctional protein produced by activated T lymphocytes. IL-3 supports the formation of multilineage colonies in the early development of multipotent hematopoietic progenitor cells. IL-3 also interacts with IL-2 to stimulate growth of T lymphocytes and induce IgG secretion from activated B cells.
Physical form
Lyophilized with no additives. Sterile filtered through a 0.2 μm filter.
Analysis Note
The biological activity is measured by the dose-dependent stimulation of the proliferation of human TF-1 cells.
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
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